Abd Aziz, Nazrin and Tan, Boon Chin and Rejab, Nur Ardiyana and Othman, Rofina Yasmin and Khalid, Norzulaani (2020) A new plant expression system for producing pharmaceutical proteins. Molecular Biotechnology, 62 (4). pp. 240-251. ISSN 1073-6085, DOI https://doi.org/10.1007/s12033-020-00242-2..
Full text not available from this repository.Abstract
In the past decade, interest in the production of recombinant pharmaceutical proteins in plants has tremendously progressed because plants do not harbor mammalian viruses, are economically competitive, easily scalable, and capable of carrying out complex post-translational modifications required for recombinant pharmaceutical proteins. Mucuna bracteata is an essential perennial cover crop species widely planted as an underground cover in oil palm and rubber plantations. As a legume, they have high biomass, thrive in its habitat, and can fix nitrogen. Thus, M. bracteata is a cost-efficient crop that shows ideal characteristics as a platform for mass production of recombinant protein. In this study, we established a new platform for the transient production of a recombinant protein in M. bracteata via vacuum-assisted agro-infiltration. Five-week-old M. bracteata plants were vacuum infiltrated with Agrobacterium tumefaciens harboring a plasmid that encodes for an anti-toxoplasma immunoglobulin (IgG) under different parameters, including trifoliate leaf positional effects, days to harvest post-infiltration, and the Agrobacterium strain used. Our results showed that vacuum infiltration of M. bracteata plant with A. tumefaciens strain GV3101 produced the highest concentration of heterologous protein in its bottom trifoliate leaf at 2 days post-infiltration. The purified anti-toxoplasma IgG was then analyzed using Western blot and ELISA. It was demonstrated that, while structural heterogeneity existed in the purified anti-toxoplasma IgG from M. bracteata, its transient expression level was two-fold higher than the model platform, Nicotiana benthamiana. This study has laid the foundation towards establishing M. bracteata as a potential platform for the production of recombinant pharmaceutical protein.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya PPP Grant [PG254-2016A], High Impact Research Programme of University of Malaya, Malaysia [UM.C/625/1/HIR/MOHE/SCI/18] |
| Uncontrolled Keywords: | Recombinant pharmaceutical protein; Mucuna bracteata; Biopharming; Transient expression |
| Subjects: | R Medicine S Agriculture > SB Plant culture |
| Divisions: | Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 05 Oct 2023 06:53 |
| Last Modified: | 05 Oct 2023 06:53 |
| URI: | http://eprints.um.edu.my/id/eprint/36869 |
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