Liu, Xinmei and Yang, Xinyuan and Shao, Juan and Hong, Yufeng and Gopinath, Subash C. B. and Chen, Yeng and Wey, Mang Chek and Wang, Yaru (2020) Coordination of nanoconjugation with an antigen/antibody for efficient detection of gynecological tumors. Journal of Analytical Methods in Chemistry, 2020. ISSN 2090-8865, DOI https://doi.org/10.1155/2020/6528572.
Full text not available from this repository.Abstract
Cervical, ovarian, and endometrial cancers are common in the female reproductive system. Cervical cancer starts from the cervix, while ovarian cancer develops when abnormal cells grow in the ovary. Endometrial or uterine cancer starts from the lining of the womb in the endometrium. Approximately 12,000 women are affected every year by cervical cancer in the United States. Squamous cell carcinoma antigen (SCC-Ag) is a well-established biomarker in serum for diagnosing gynecological cancers, and its levels were observed to be elevated in cervical, ovarian, and endometrial cancer patients. Moreover, SCC-Ag was used to identify the tumor size and progression stages. Various biosensing systems have been proposed to identify SCC-Ag; herein, enhanced interdigitated electrode sensing is presented with the use of gold nanoparticles (GNPs) to conjugate an antigen/antibody. It was proved that the limit of detection is 62.5 fM in the case of antibody-GNP, which is 2-fold higher than that by SCC-Ag-GNP. Furthermore, the antibody-GNP-modified surface displays greater current increases with concomitant dose-dependent SCC-Ag levels. High analytical performance was shown by the discrimination against alpha-fetoprotein and CYFRA 21-1 at 1 pM. An enhanced sensing system is established for gynecological tumors, representing an advance from the earlier detection methods.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Cell carcinoma antigen; Colorimetric detection |
| Subjects: | R Medicine |
| Divisions: | Faculty of Dentistry |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 04 Oct 2023 07:05 |
| Last Modified: | 04 Oct 2023 07:05 |
| URI: | http://eprints.um.edu.my/id/eprint/36804 |
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