Hosseini, Samira and Azari, Pedram and Cardenas-Benitez, Braulio and Martinez-Guerra, Eduardo and Aguirre-Tostado, Francisco S. and Vazquez-Villegas, Patricia and Pingguan-Murphy, Belinda and Madou, Marc J. and Martinez-Chapa, Sergio O. (2020) A LEGO inspired fiber probe analytical platform for early diagnosis of Dengue fever. Materials Science and Engineering C-Materials for Biological Applications, 109. ISSN 09284931, DOI https://doi.org/10.1016/j.msec.2020.110629.
Full text not available from this repository.Abstract
Based on the concept of LEGO toys, a fiber probe analytical platform (FPAP) was developed as a powerful diagnostic tool offering higher sensitivity in detection of infectious agents compared to established methods. Using the form and the function of LEGO toys, this protocol describes a fiber-based, 96-well plate, which suspends a new class of chemically-designed, electrospun fibers within the assay. This clamping strategy allows both sides of the developed fiber mats to interact with biomolecules within the assay thus benefiting from the tailored chemical and physical properties of these fiber-based bioreceptors in attracting the biomolecules to the surface. The fabrication method of FPAP involves one-step electrospinning of the chemically designed fibers, 3D printing of the LEGO-like probing segments, and assembly of the device followed by ELISA procedure. FPAP follows the same principles of operation as that of a conventional enzyme linked immunosorbent assay (ELISA), therefore, it can be run by lab technicians, expert in ELISA. FPAP was used for early diagnosis of Dengue fever and provided an 8-fold higher sensitivity while the limit of detection (LOD) was recorded to be in femto-gram per milliliter range which is significantly low when compared to other existing techniques or conventional assay. This platform allows different types of paper/fiber bio-receptive platforms to be incorporated within the design that promises simultaneous recognition of multiple infectious agents.
| Item Type: | Article |
|---|---|
| Funders: | Nano Sensors and Devices Focus Group, School of Engineering and Sciences, Tecnologico de Monterrey [Grant No: 002EICII01], Ministry of Higher Education, Malaysia, Frontiers Foundation [Grant No: FG021-17AFR], Instituto Tecnológico y de Estudios Superiores de Monterrey |
| Uncontrolled Keywords: | Paper-/fiber-based analytical platforms; Dengue detection; Surface functional groups; Integrated bio-sensing platform; Electrospinning |
| Subjects: | R Medicine > R Medicine (General) > Medical technology |
| Divisions: | Faculty of Engineering Faculty of Engineering > Biomedical Engineering Department |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 06 Nov 2024 01:27 |
| Last Modified: | 06 Nov 2024 01:27 |
| URI: | http://eprints.um.edu.my/id/eprint/36783 |
Actions (login required)
 |
View Item |
