Lai, Meng Yee and Lau, Yee Ling (2020) Detection of Plasmodium knowlesi using recombinase polymerase amplification (RPA) combined with SYBR Green I. Acta Tropica, 208. ISSN 0001706X, DOI https://doi.org/10.1016/j.actatropica.2020.105511.
Full text not available from this repository.Abstract
In this study, recombinase polymerase amplification (RPA) combined with SYBR Green I was developed for the detection of Plasmodium knowlesi. Positive samples were indicated with a green color while negative samples were orange. To increase the efficiency of amplification, an interval mixing step of samples after 3 to 6 min incubation was recommended. Different sets of reaction volumes from 6.25 to 50 mu L were tested and the results indicated no differences in detection. RPA's combination with SYBR Green I is fast and easy to perform, hence this method is suitable for use in resource-limited settings.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education, Malaysia (Grant No. LR002D-2018) |
| Uncontrolled Keywords: | P. knowlesi; Recombinase polymerase amplification; Isothermal; SYBR Green I; Molecular detection |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 30 Dec 2023 16:22 |
| Last Modified: | 30 Dec 2023 16:22 |
| URI: | http://eprints.um.edu.my/id/eprint/36518 |
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