Tan, Y. H. and Alias, Z. (2020) Expression of cytosolic and thiolated proteome of Musca domestica larvae under oxidative challenge. Tropical Biomedicine, 37 (3). pp. 744-755. ISSN 01275720,
Full text not available from this repository.Abstract
The study was aimed to investigate the expression of cytosolic and thiolated proteins of Musca domestica larvae under oxidative stress. Proteins from acute treatment of hydrogen peroxide (LC50 = 21.52% (v/v)) on 3rd stage larvae of housefly were extracted and purified using an activated Thiol Sepharose (R) for thiolated protein purification. Two dimensional gel electrophoresis was used for visualizing and analyzing expression of cytosolic and thiolated proteins. Protein spots with more than 5 fold of expression change were identified using liquid chromatography- tandem mass spectrometry (LC-MS/MS). The cytosolic proteins were actin, tropomyosin, ubiquitin, arginine kinase, pheromone binding protein/general odorant binding protein, and ATP: guanidino phosphotransferase. The thiolated proteins with more than 5 fold change in expression as an effect to the acute treatment were fructose bisphosphate aldolase, short chain dehydrogenase and lactate/malate dehydrogenase. The proteins identified in the study should provide vital information for future reference in oxidative stress defence and response occurring in houseflies.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education [Grant No: FP052-2014A], University of Malaya Postgraduate Research Fund [Grant No: PPP: PV091/2011A)] |
| Uncontrolled Keywords: | Animals; Cytosol; Houseflies; Larva; Lipid Peroxidation; Oxidative Stress; Proteome |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 28 Oct 2024 13:10 |
| Last Modified: | 28 Oct 2024 13:10 |
| URI: | http://eprints.um.edu.my/id/eprint/36469 |
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