Ansari, Abdul W. and Ahmad, Fareed and Shankar, Esaki M. and Yong, Yean K. and Tan, Hong Y. and Jacobs, Roland and Larsson, Marie and Velu, Vijayakumar and Kamarulzaman, Adeeba and Schmidt, Reinhold E. (2020) Brief report: Diminished coinhibitory molecule 2B4 xxpression is associated with preserved iNKT cell phenotype in HIV long-term nonprogressors. Jaids-Journal of Acquired Immune Deficiency Syndromes, 85 (1). pp. 73-78. ISSN 1525-4135, DOI https://doi.org/10.1097/QAI.0000000000002399.
Full text not available from this repository.Abstract
Background: We have previously shown an association of elevated coinhibitory molecule 2B4 expression with iNKT cells alterations in HIV disease. Herein, we show a comparative analysis of 2B4 expression on iNKT cells of HIV long-term nonprogressors (LTNPs) and progressors. Methods: Antiretroviral therapy-naive HIV-seropositive individuals (progressors, n = 16) and LTNPs (n = 10) were recruited for this study. We used multicolor flow cytometry on frozen peripheral blood mononuclear cells to determine iNKT subset frequencies, the levels of coinhibitory 2B4 expression, and intracellular interferon-gamma (IFN-gamma) production. CD1d tetramer was used to characterize iNKT cells. Results: We report significantly lower level of 2B4 expression on bulk LTNPs iNKT cells and on their CD4 subsets compared with HIV progressors. Furthermore, the iNKT cells from LTNPs produced higher amount of IFN-gamma than HIV progressors as detected by intracellular cytokine staining. Interestingly, the frequency of 2B4(+)iNKT cells of progressors but not LTNPs significantly correlates with CD4 T-cell count, HIV viral load, and IFN-gamma(+)production by iNKT cells. Conclusion: Our results suggest that in addition to suppressed HIV replication, diminished 2B4 expression and associated coinhibitory signaling, and substantial production of IFN-gamma could contribute to preserved iNKT cell phenotype in LTNPs.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | iNKT cells; CD4; HIV; LTNP; IFN-gamma |
| Subjects: | R Medicine R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Faculty of Medicine Faculty of Medicine > Medicine Department |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 30 Nov 2023 05:57 |
| Last Modified: | 30 Nov 2023 05:57 |
| URI: | http://eprints.um.edu.my/id/eprint/36418 |
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