Lau, Yee Ling and Ismail, Ilyiana and Mustapa, Nur and Lai, Meng and Soh, Tuan and Hassan, Afifah and Peariasamy, Kalaiarasu and Lee, Yee and Goh, Pik (2020) A sensitive reverse transcription loop-mediated isothermal amplification assay for direct visual detection of SARS-CoV-2. American Journal of Tropical Medicine and Hygiene, 103 (6). pp. 2350-2352. ISSN 0002-9637, DOI https://doi.org/10.4269/ajtmh.20-1079.
Full text not available from this repository.Abstract
A simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Rapid detection |
| Subjects: | R Medicine R Medicine > R Medicine (General) > Medical technology R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Faculty of Medicine Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 29 Nov 2023 05:54 |
| Last Modified: | 29 Nov 2023 05:54 |
| URI: | http://eprints.um.edu.my/id/eprint/36222 |
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