The production of functional γ-aminobutyric acid Malaysian soy sauce koji and moromi using the trio of Aspergillus oryzae NSK, Bacillus cereus KBC, and the newly identified Tetragenococcus halophilus KBC in liquid-state fermentation

Shin Yee, C. and Sohedein, M.N.A. and Poh Suan, O. and Weng Loen, A.W. and Abd Rahim, M.H. and Soumaya, S. and Ilham, Z. and Wan-Mohtar, W.A.A.Q.I. (2021) The production of functional γ-aminobutyric acid Malaysian soy sauce koji and moromi using the trio of Aspergillus oryzae NSK, Bacillus cereus KBC, and the newly identified Tetragenococcus halophilus KBC in liquid-state fermentation. Future Foods, 4. ISSN 26668335, DOI https://doi.org/10.1016/j.fufo.2021.100055.

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Abstract

Soy sauce fermentation was improved using the trio of A. oryzae (AO), B. cereus (BC), and the newly identified Tetragenococcus halophilus KBC (TH) in liquid-state fermentation. The effect of molasses (5, 10, and 20) and type of wheat flour (organic-WFO and commercial-WFC) were tested on the koji-moromi formulation for efficient ɣ-aminobutyric acid (GABA) production. The koji-WFO produced higher GABA (1290 mg/L) compared to koji-WFC (13 mg/L). In the initial moromi mixture, 2 × 10−4 mL of BC inoculum containing 5–20 of molasses was added and replaced the same amount with TH in the second trial. Subsequently, the moromi was mixed with 1 × 10−4 mL of BC and TH, which shortened the 60-day moromi process to 30 days. Meanwhile, BC-moromi cultured in 20 molasses generated significantly lower GABA (118 mg/L) production compared to other percentages, however the concentration boosted for TH-moromi in 20 molasses (159 mg/L). The combination of BC-TH percentages (5–20) gave significantly lower GABA (83–137 mg/L) than the singular 20 TH treatment. The results promote the use of TH in producing high-GABA soy sauce. © 2021

Item Type: Article
Funders: GPF Geran Penyelidikan Fakulti (Grant No. GPF084A-2020), SATU Joint Research Scheme Program Universiti Malaya (Grant No. ST006-2019), Ministry of Higher Education, Malaysia
Uncontrolled Keywords: GABA; Molasses; Soy sauce; Tetragenococcus halophilus; Wheat flour
Subjects: S Agriculture > S Agriculture (General)
Divisions: Faculty of Medicine > School of Biological Sciences
Depositing User: Ms Zaharah Ramly
Date Deposited: 20 Dec 2023 14:25
Last Modified: 20 Dec 2023 14:25
URI: http://eprints.um.edu.my/id/eprint/35928

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