Ilias, A.N. and Ismail, I.S. and Hamzah, H. and Mohd Mohidin, Taznim Begam and Idris, M.F. and Ajat, M. (2021) Rebaudioside A enhances LDL cholesterol uptake in HepG2 cells via suppression of HMGCR expression. Reports of Biochemistry and Molecular Biology, 10 (3). pp. 401-411. ISSN 2322-3480,
Full text not available from this repository.Abstract
Background: Rebaudioside A is one of the major diterpene glycosides found in Stevia had been reported to possess anti-hyperlipidemic effects. In this study, we explore the potential cholesterol-regulating mechanisms of Rebaudioside A in the human hepatoma (HepG2) cell line in comparison with simvastatin. Methods: Cells were incubated with Rebaudioside A at several concentrations (0-10 μM) to determine the cytotoxicity by the MTT assay. Cells were treated with selected dosage (1 and 5 μM) in further experiments. Total cellular lipid was extracted by Bligh and Dyer method and subjected to quantitative colorimetric assay. To illustrate the effect of Rebaudioside A on cellular lipid droplets and low-density lipoprotein receptors, treated cells were subjected to immunofluorescence microscopy. Finally, we investigated the expression of experimental gene patterns of cells in response to treatment. Results: In this study, cytotoxicity of Rebaudioside A was determined at 27.72 μM. Treatment of cells with a higher concentration of Rebaudioside A promotes better hepatocellular cholesterol internalization and ameliorates cholesterol-regulating genes such as HMGCR, LDLR, and ACAT2. Conclusions: In conclusion, our data demonstrated that Rebaudioside A is capable to regulate cholesterol levels in HepG2 cells. Hence, we proposed that Rebaudioside A offers a potential alternative to statins for atherosclerosis therapy. © 2021. Reports of Biochemistry and Molecular Biology. All Rights Reserved.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Putra Malaysia [Grant No: Geran Putra: GP/2020/9693000], Faculty of Veterinary Medicine (UPM) |
| Uncontrolled Keywords: | Rebaudioside A; Anti-hypercholesterolemia; Lipid droplets; Low-density lipoprotein; HMGCR |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 27 Nov 2023 07:07 |
| Last Modified: | 27 Nov 2023 07:07 |
| URI: | http://eprints.um.edu.my/id/eprint/35876 |
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