Comparison of alcohol shock enrichment and selective enrichment for the isolation of Clostridium difficile

Riley, T.V.; Brazier, J.S.; Hassan, H.; Williams, K.; Phillips, K.D. (1987) Comparison of alcohol shock enrichment and selective enrichment for the isolation of Clostridium difficile. Epidemiology and Infection, 99 (02). pp. 355-359. ISSN 1469-4409

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    Abstract

    Two enrichment methods were compared for their ability to recover Clostridium difficile from stool samples. One method used selective enrichment in an antibiotic-containing broth followed by detection with a latex particle agglutination (LPA) reagent. The other used enrichment in a non-selective broth following treatment of the specimen with alcohol. With clinical specimens enrichment culture was significantly more successful at detecting C. difficile than direct plating. Alcohol shock enrichment was twice as effective as direct culture, while selective broth enrichment was three times more effective. The use of LPA for screening selective enrichment broths for C. difficile should prove a cost-effective measure as only positive broths (about 20) require subculture for confirmation.

    Item Type: Article
    Creators:
    1. Riley, T.V.
    2. Brazier, J.S.
    3. Hassan, H.(Department of Medical Microbiology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    4. Williams, K.
    5. Phillips, K.D.
    Journal or Publication Title: Epidemiology and Infection
    Additional Information: Department of Medical Microbiology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
    Uncontrolled Keywords: Medical Microbiology
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: hamimah hamimah hassan
    Date Deposited: 01 Aug 2012 16:02
    Last Modified: 11 Dec 2013 11:33
    URI: http://eprints.um.edu.my/id/eprint/3583

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