Detection of clostridium difficile from an enhancement broth by gas-liquid chromatography

Hassan, H.; Cheng, S.C. (1991) Detection of clostridium difficile from an enhancement broth by gas-liquid chromatography. The Malaysian journal of pathology, 13 (1). pp. 29-31. ISSN 0126-8635

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    Gas-Liquid cl~romatograpl~y (GLC) was used to detect tlle presence of isocaproic acid produced by Clostridium difficile from 54 stool san~ples grown in cycloserine-cefoxitin brotli. Isocaproic acid was detected in 12 samples of which 5 were confirmed to be Clostridium difficile by culture and biocllemical tests. Tlle detection of isocaproic acid by GLC together with the presence of presumptive colonies on primary selective culture media provides a more rapid laboratory diagnosis for Clostridium difficile.

    Item Type: Article
    1. Hassan, H.(Department of Medical Microbiology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    2. Cheng, S.C.
    Journal or Publication Title: The Malaysian journal of pathology
    Additional Information: Department of Medical Microbiology, Faculty o f Medicine, University o f Malaya, Kuala Lumpur.
    Uncontrolled Keywords: Gas-Liquid cl~romatograpl~y (GLC); Medical Microbiology
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: hamimah hamimah hassan
    Date Deposited: 02 Aug 2012 10:20
    Last Modified: 02 Aug 2012 10:20

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