Mohd Bukhari, Fatma Diyana and Lau, Yee Ling and Fong, Mun Yik (2021) Erythrocyte binding activity of pkdbpαii of plasmodium knowlesi isolated from high and low parasitemia cases. American Journal of Tropical Medicine snd Hygiene, 104 (2). pp. 680-682. ISSN 0002-9637, DOI https://doi.org/10.4269/ajtmh.20-0797.
Full text not available from this repository.Abstract
Invasion of Plasmodium knowlesi merozoite into human erythrocytes involves molecular interaction between the parasite's Duffy binding protein (PkDBPαII) and the Duffy antigen receptor for chemokines on the erythrocytes. This study investigates the binding activity of human erythrocyte with PkDBPαII of P. knowlesi isolates from high and low parasitemic patients in an erythrocyte binding assay. The binding activity was determined by counting the number and measuring the size of rosettes formed in the assay. The protein PkDBPαII of P. knowlesi isolated from low parasitemia cases produced significantly higher number of rosettes with human erythrocytes than high parasitemia case isolates (65.5 ± 12.9 and 17.2 ± 5.5, respectively). Interestingly, PkDBPαII of isolates from high parasitemia cases formed significantly larger rosettes with human erythrocytes than PkDBPαII of isolates from low parasitemia cases (18,000 ± 13,000 μm2 and 1,315 ± 623 μm2, respectively). © 2021 by The American Society of Tropical Medicine and Hygiene
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education, Malaysia, Universiti Malaya [Grant No: LR002A-2018 & LRGS 1/2018] |
| Uncontrolled Keywords: | Invasion; Human; Molecular interatcion; Parasitemia; Binding |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 30 Oct 2023 07:42 |
| Last Modified: | 30 Oct 2023 07:42 |
| URI: | http://eprints.um.edu.my/id/eprint/35682 |
Actions (login required)
 |
View Item |
