Alkaline phosphatase activity assessment Of two endodontic materials: a preliminary study

Rajan, S. and Awang, H. and Devi, S. and PooP, A.H. and Hassan, H. (2008) Alkaline phosphatase activity assessment Of two endodontic materials: a preliminary study. Annals of Dentistry, 15 (1). pp. 5-10. ISSN 0128-7532,

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Abstract

Objective: An in vitro assessment of MG-63 human osteosarcoma cells' alkaline phosphatase (ALP) activity when in contact with calcium hydroxide powder (CH), paste (CHP) and grey mineral trioxide aggregate (MTA). Methods: MG-63 cells were seeded to the three selected materials for durations of 0.25, 0.5, 1, 24, 48 and 72 hours. BCIP-NBT assay was used and ALP activity quantified using ELISA reader at 410 nm. Results: The overall analysis for ALP activity indicated significant interaction between test materials and control (maintenance medium). Subsequently, the test materials were paired and analysed for initial (0.25, 0.5, 1 hour) and delayed response (24, 48 and 72 hours). During the initial response, CH exhibited an increased ALP activity compared to MTA. This interaction was not dependant on duration. The delayed response exhibited elevated ALP activity with CHP when compared to MTA and CH. The interaction of CHP was dependant on duration. Conclusion: All three materials exhibited increased ALP activity.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Department of Medical Microbiology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords:	Key words: MTA, calcium hydroxide, illkaline phosphatase, in vitro assessment, MG-63 cell line
Subjects:	R Medicine
Divisions:	Faculty of Medicine
Depositing User:	hamimah hamimah hassan
Date Deposited:	06 Aug 2012 01:35
Last Modified:	06 Aug 2012 01:35
URI:	http://eprints.um.edu.my/id/eprint/3553

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