Balamurugan, Joshini Pillai and Supramani, Sugenendran and Usuldin, Siti Rokhiyah Ahmad and Ilham, Zul and Klaus, Anita and Ikram, Nur Kusaira Khairul and Ahmad, Rahayu and Wan-Mohtar, Wan Abd Al Qadr Imad (2021) Efficient biomass-endopolysaccharide production from an identified wild-Serbian Ganoderma applanatum strain BGS6Ap mycelium in a controlled submerged fermentation. Biocatalysis and Agricultural Biotechnology, 37. ISSN 1878-8181, DOI https://doi.org/10.1016/j.bcab.2021.102166.
Full text not available from this repository.Abstract
A rare wild-Serbian Ganoderma applanatum strain BGS6Ap (GASB) was morphologically identified based on its basidiocarp with brownish-red cap and woody stipe. Molecularly, GASB DNA was extracted, sequenced (651 bp) and subjected to phylogenetic analysis with evolutionary distance (K-nuc). A plasmid Editor (ApE) software was used to verify the sequence and GASB was found to be 99% similarity to the commercial G. applanatum (GA) ATCC44053. The production of GASB's biomass and endopolysaccharide (ENS) in different temperature was screened at 20 degrees C, 25 degrees C and 30 degrees C via one-factor-at-a-time (OFAAT) scheme in a submerged liquid fermentation (SLF). Lower temperature was chosen due to GASB's natural habitat, which belongs to temperate continental climates with the influence of air currents from the Carpathians and the Mediterranean. Using the OFAAT data, the SLF was further optimised using response surface methodology (RSM) to generate biomass-ENS based on temperature (20 degrees C-30 degrees C), agitation rate (100-200 rpm), glucose concentration (10 g/L-50 g/L) and initial pH (4-6). The highest production of biomass (17.51 g/L) and ENS (3.47 g/L) was generated using optimal conditions of 25 degrees C, 150 rpm, 10 g/L of glucose and pH 6. To study the structural composition, ENS confirmed spectral linkages in FTIR (2925 cm(-1), 1623 cm(-1), 1073 cm(-1), 890 cm(-1)) and NMR (delta 4.30, 4.80, 5.20 ppm) as endo-beta-D-glucan. This approach could serve as a blueprint for novel mycelial biomass and endo-beta-D-glucan production of GASB which can be further studied on different temperate basidiomycetes.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya under the Research University Grant-Faculty Program [GPF084A-2020], Ministry of Higher Education & Scientific Research (MHESR) [IIRG003A-2020IISS] |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 26 May 2022 03:43 |
| Last Modified: | 26 May 2022 03:43 |
| URI: | http://eprints.um.edu.my/id/eprint/34903 |
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