See, SiouNing Aileen and Goh, Zee Hong and Chan, Yen Yew and Chong, Khai En and Tan, Geok Yuan Annie and Bhassu, Subha and Othman, Rofina Yasmin (2021) Biosafety evaluation and detection of shrimp viruses on field samples using dual priming oligonucleotide (DPO) system based multiplex PCR assay. Gene Reports, 23. ISSN 2452-0144, DOI https://doi.org/10.1016/j.genrep.2021.101158.
Full text not available from this repository.Abstract
Disease is the current major challenge and issue in shrimp aquaculture as it can be spread easily causing global outbreak. The risk of disease outbreak in shrimp populations can be further minimized with rapid and accurate diagnostic method. This study aims to develop a dual priming oligonucleotide (DPO) based multiplex PCR system for simultaneous detection of shrimp DNA and RNA viruses. This system targets Baculovirus Penaei (BP), Monodon Baculovirus (MB), Infectious Hypodermal, Haematopoietic Necrosis Virus (IHHNV) and White Spot Syndrome Virus (WSSV), Macrobrachium rosenbergii nodavirus (MrNV), Infectious Myonecrosis Virus (IMNV), Taura Syndrome Virus (TSV) and Yellow Head Virus (YHV). The shrimp samples collected from shrimp farm were diagnosed to be infected with YHV infection in the 2nd cycle in year 2018 with 80% screening rate (4/5). DPO based multiplex PCR system may facilitate the rapid and effective screening of shrimp viruses in laboratory.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya Centre of Innovation and Commercialization[(UMCIC) PV019-2016], Sub-Program 18 under CEBAR Research Programs entitled "Biosecurity and Biosafety for Tropical Agricultural Bioeconomy"[RU2017], Sub-Program 18 under CEBAR Research Programs entitled "Biosecurity and Biosafety for Tropical Agricultural Bioeconomy"[RU006-2017] |
| Uncontrolled Keywords: | Shrimp viruses;Dual priming oligonucleotide DPO;Biosafety evaluation |
| Subjects: | S Agriculture > SH Aquaculture. Fisheries. Angling |
| Divisions: | Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 28 May 2022 04:58 |
| Last Modified: | 28 May 2022 04:58 |
| URI: | http://eprints.um.edu.my/id/eprint/34829 |
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