Bioassay-guided isolation of cytotoxic cycloartane triterpenoid glycosides from the traditionally used medicinal plant Leea Indica

Wong, Y.H.; Abdul Kadir, H.; Ling, S.K. (2012) Bioassay-guided isolation of cytotoxic cycloartane triterpenoid glycosides from the traditionally used medicinal plant Leea Indica. Evid Based Complement Alternat Med.

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    Abstract

    Leea indica is a medicinal plant used traditionally to cure cancer. In this study, the cytotoxic compounds of L. indica were isolated using bioassay-guided approach. Two cycloartane triterpenoid glycosides, mollic acid arabinoside (MAA) and mollic acid xyloside (MAX), were firstly isolated from L. indica. They inhibited the growth of Ca Ski cervical cancer cells with IC(50) of 19.21 μM (MAA) and 33.33 μM (MAX). MRC5 normal cell line was used to calculate selectivity index. MAA and MAX were about 8 and 4 times more cytotoxic to Ca Ski cells compared to MRC5. The cytotoxicity of MAA was characterized by both cytostatic and cytocidal effects. MAA decreased the expression of proliferative cell nuclear antigen, increased sub-G1 cells, and arrested cells in S and G2/M phases. This study provides the evidence for the ethnomedicinal use of L. indica and paves the way for future mechanism studies on the anticancer effects of MAA.

    Item Type: Article
    Creators:
    1. Wong, Y.H.
    2. Abdul Kadir, H.
    3. Ling, S.K.
    Journal or Publication Title: Evid Based Complement Alternat Med
    Additional Information: Biomolecular Research Group, Biochemistry Division, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ---> Correspondence should be addressed to Habsah Abdul Kadir, aakhak@yahoo.com
    Uncontrolled Keywords: Leea Indica; Biochemistry
    Subjects: R Medicine
    Divisions: Faculty of Science > Institute of Biological Sciences
    Depositing User: Mr. Faizal Hamzah
    Date Deposited: 04 Jul 2012 14:22
    Last Modified: 04 Jul 2012 14:22
    URI: http://eprints.um.edu.my/id/eprint/3471

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