Lignosus rhinocerus (cooke) ryvarden: a medicinal mushroom that stimulates neurite outgrowth in pc-12 cells

Eik, L.F.; Naidu, M.; David, P.; Wong, K.H.; Tan, Y.S.; Sabaratnam, V. (2012) Lignosus rhinocerus (cooke) ryvarden: a medicinal mushroom that stimulates neurite outgrowth in pc-12 cells. Evid Based Complement Alternat Med..

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    Abstract

    A national treasure mushroom, Lignosus rhinocerus, has been used to treat variety of ailments by local and indigenous communities in Malaysia. The aim of this study was to investigate the potential of the most valuable part of L. rhinocerus, the sclerotium, on neurite outgrowth activity by using PC-12Adh cell line. Differentiated cells with one thin extension at least double the length of the cell diameter were scored positive. Our results showed that aqueous sclerotium L. rhinocerus extract induced neurite outgrowths of 24.4% and 42.1% at 20 μg/mL (w/v) of aqueous extract alone and a combination of 20 μg/mL (w/v) aqueous extract and 30 ng/mL (w/v) of NGF, respectively. Combination of NGF and sclerotium extract had additive effects and enhanced neurite outgrowth. Neuronal differentiation was demonstrated by indirect immunofluorescence of neurofilament protein. Aqueous sclerotium extract contained neuroactive compounds that stimulated neurite outgrowth in vitro. To our knowledge this is the first report on neurite-stimulating activities of L. rhinocerus.

    Item Type: Article
    Creators:
    1. Eik, L.F.
    2. Naidu, M.
    3. David, P.
    4. Wong, K.H.
    5. Tan, Y.S.
    6. Sabaratnam, V.
    Journal or Publication Title: Evid Based Complement Alternat Med.
    Additional Information: Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia -----> Correspondence should be addressed to Vikineswary Sabaratnam, viki@um.edu.my
    Uncontrolled Keywords: Mushroom
    Subjects: R Medicine
    Divisions: UNSPECIFIED
    Depositing User: Mr. Faizal Hamzah
    Date Deposited: 04 Jul 2012 09:51
    Last Modified: 11 Dec 2013 15:51
    URI: http://eprints.um.edu.my/id/eprint/3470

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