Induction of Mitochondria-Mediated apoptosis in Ca ski human cervical cncer cells triggered by mollic acid arabinoside isolated from Leea indica

Wong, Y.H.; Abdul Kadir, H. (2012) Induction of Mitochondria-Mediated apoptosis in Ca ski human cervical cncer cells triggered by mollic acid arabinoside isolated from Leea indica. Evid Based Complement Alternat Med.

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    Abstract

    Leea indica is a medicinal plant traditionally used to treat cancer. Through bioassay-guided approach, we isolated mollic acid arabinoside (MAA), for the first time from Leea indica. Here, we present the apoptosis-inducing effect of MAA on Ca Ski cervical cancer cells. Based on DAPI staining, MAA-treated cells manifested nuclear shrinkage, condensation, and fragmentation. We further confirmed the fragmentation of DNA using TUNEL assay. During early apoptosis, MAA caused the perturbation of plasma membrane through externalization of PS, followed by the formation of apoptotic blebs. Prior to these events, MAA triggered rapid dissipation of the mitochondrial membrane potential. In the upstream, MAA increased the expression of Bax, decreased the expression of Bcl-2, and augmented the Bax/Bcl-2 ratio. These findings suggested that MAA induced mitochondrial-mediated apoptosis in Ca Ski cells and thus provide the scientific explanation for the traditional application of this herbal medicine in cancer treatment.

    Item Type: Article
    Journal or Publication Title: Evid Based Complement Alternat Med
    Additional Information: Biomolecular Research Group, Biochemistry Program, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia----> Correspondence should be addressed to Habsah Abdul Kadir, aakhak@yahoo.com
    Uncontrolled Keywords: Biological Sciences
    Subjects: R Medicine
    Divisions: Faculty of Science > Institute of Biological Sciences
    Depositing User: Mr. Faizal Hamzah
    Date Deposited: 04 Jul 2012 09:39
    Last Modified: 04 Jul 2012 09:39
    URI: http://eprints.um.edu.my/id/eprint/3469

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