Sriram, Sandhya and Kang, Nam-Young and Subramanian, Subha and Nandi, Tannistha and Sudhagar, Samydurai and Xing, Qiaorui and Tong, Gerine Jin-Ling and Chen, Allen Kuan-Liang and Srijaya, Thekkeparambil Chandrabose and Tan, Patrick and Loh, Yuin-Han and Chang, Young-Tae and Sugii, Shigeki (2021) Novel live cell fluorescent probe for human-induced pluripotent stem cells highlights early reprogramming population. Stem Cell Research and Therapy, 12 (1). ISSN 1757-6512, DOI https://doi.org/10.1186/s13287-021-02171-6.
Full text not available from this repository.Abstract
Background Despite recent rapid progress in method development and biological understanding of induced pluripotent stem (iPS) cells, there has been a relative shortage of tools that monitor the early reprogramming process into human iPS cells. Methods We screened the in-house built fluorescent library compounds that specifically bind human iPS cells. After tertiary screening, the selected probe was analyzed for its ability to detect reprogramming cells in the time-dependent manner using high-content imaging analysis. The probe was compared with conventional dyes in different reprogramming methods, cell types, and cell culture conditions. Cell sorting was performed with the fluorescent probe to analyze the early reprogramming cells for their pluripotent characteristics and genome-wide gene expression signatures by RNA-seq. Finally, the candidate reprogramming factor identified was investigated for its ability to modulate reprogramming efficiency. Results We identified a novel BODIPY-derived fluorescent probe, BDL-E5, which detects live human iPS cells at the early reprogramming stage. BDL-E5 can recognize authentic reprogramming cells around 7 days before iPS colonies are formed and stained positive with conventional pluripotent markers. Cell sorting of reprogrammed cells with BDL-E5 allowed generation of an increased number and higher quality of iPS cells. RNA sequencing analysis of BDL-E5-positive versus negative cells revealed early reprogramming patterns of gene expression, which notably included CREB1. Reprogramming efficiency was significantly increased by overexpression of CREB1 and decreased by knockdown of CREB1. Conclusion Collectively, BDL-E5 offers a valuable tool for delineating the early reprogramming pathway and clinically applicable commercial production of human iPS cells.
| Item Type: | Article |
|---|---|
| Funders: | Development Programme Grant by A*STAR's Joint Council Office [1334 k00083], Ministry of Education, Malaysia [UM.C/HIR/MOHE/DENT/01] [UMRG RP019/13HTM] |
| Uncontrolled Keywords: | DOFLA library fluorescence dye; Human induced pluripotent stem cell (hiPSC); Early stage pluripotency; Mesenchymal-epithelial transition (MET); Adipose-derived stromal cell (ASC); Dental pulp stem cell (DPSC); Golgi marker; Three-dimensional (3D) microcarrier-based culture system; cAMP responsive element binding protein (CREB) |
| Subjects: | Q Science > QH Natural history > QH301 Biology R Medicine > R Medicine (General) |
| Divisions: | Faculty of Dentistry |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 14 Sep 2022 07:48 |
| Last Modified: | 14 Sep 2022 07:48 |
| URI: | http://eprints.um.edu.my/id/eprint/34567 |
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