Lai, Meng Yee and Bukhari, Fatma Diyana Mohd and Zulkefli, Nur Zulaikha and Ismail, Ilyiana and Mustapa, Nur Izati and Soh, Tuan Suhaila Tuan and Hassan, Afifah Haji and Peariasamy, Kalaiarasu M. and Lee, Yee Leng and Suppiah, Jeyanthi and Thayan, Ravindran and Lau, Yee Ling (2021) Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2. BMC Infectious Diseases, 21 (1). ISSN 1471-2334, DOI https://doi.org/10.1186/s12879-021-06876-0.
Full text not available from this repository.Abstract
Current assays for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rely on time consuming, costly and laboratory based methods for virus isolation, purification and removing inhibitors. To address this limitation, we propose a simple method for testing RNA from nasopharyngeal swab samples that bypasses the RNA purification step. Methods In the current project, we have described two extraction-free reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for the detection of SARS-CoV-2 by using E gene and RdRp gene as the targets. Results Here, results showed that reverse transcription loop-mediated isothermal amplification assays with 88.4% sensitive (95% CI: 74.9-96.1%) and 67.4% sensitive (95% CI: 51.5-80.9%) for E gene and RdRp gene, respectively. Conclusion Without the need of RNA purification, our developed RT-LAMP assays for direct detection of SARS-CoV-2 from nasopharyngeal swab samples could be turned into alternatives to qRT-PCR for rapid screening.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Malaysia[PR001-2020B] |
| Uncontrolled Keywords: | SARS-CoV-2;Isothermal detection;Coronaviruses;Rapid diagnosis;RT-LAMP |
| Subjects: | Q Science > QR Microbiology R Medicine R Medicine > RB Pathology > Theories of disease. Etiology. Pathogenesis |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 15 Sep 2022 02:12 |
| Last Modified: | 15 Sep 2022 02:12 |
| URI: | http://eprints.um.edu.my/id/eprint/34531 |
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