Wulandari, Pugar Arga Cristina and Ilmi, Zulfa Nailul and Husen, Saikhu Akhmad and Winarni, Dwi and Alamsjah, Mochammad Amin and Awang, Khalijah and Vastano, Marco and Pellis, Alessandro and MacQuarrie, Duncan and Pudjiastuti, Pratiwi (2021) Wound healing and antioxidant evaluations of alginate from sargassum ilicifolium and mangosteen rind combination extracts on diabetic mice model. Applied Sciences-Basel, 11 (10). ISSN 2076-3417, DOI https://doi.org/10.3390/app11104651.
Full text not available from this repository.Abstract
A diabetic foot ulcer is an open wound that can become sore and frequently occurs in diabetic patients. Alginate has the ability to form a hydrophilic gel when in contact with a wound surface in diabetic patients. Xanthones are the main compounds of mangosteen rind and have antibacterial and anti-inflammatory properties. The purpose of this research was to evaluate the wound healing and antioxidants assay with a combination of alginate from S. ilicifolium and mangosteen rind combination extracts on a diabetic mice model. The characterization of alginate was carried out by size exclusion chromatography with multiple angle laser light scattering (SEC-MALLS) and thermogravimetric analysis (TGA). The M/G ratio of alginate was calculated by using proton nuclear magnetic resonance (H-1 NMR). The antioxidant activity of mangosteen rind and the combination extracts was determined using the DPPH method. The observed parameters were wound width, number of neutrophils, macrophages, fibrocytes, fibroblasts, and collagen densities. The 36 male mice were divided into 12 groups including non-diabetic control (NC), diabetes alginate (DA), alginate-mangosteen (DAM), and diabetes control (DC) groups in three different groups by a histopathology test on skin tissue. The treatment was carried out for 14 days and mice were evaluated on Days 3, 7, and 14. The SEC-MALLS results showed that the molecular weight and dispersity index (D) of alginate were 2.77 x 10(4) Dalton and 1.73, respectively. The M/G ratio of alginate was 0.77 and described as single-stage decomposition based on TGA. Alginate, mangosteen rind extract, and their combination were divided into weak, medium, and strong antioxidant, respectively. The treatment of the DA and DAM groups showed a decrease in wound width and an increase in the number of fibrocytes, fibroblasts, and macrophages. The number of neutrophils decreased while the percentage of collagen densities increased for all the considered groups.
| Item Type: | Article |
|---|---|
| Funders: | Innovation and Research Center, Airlangga University[1408/UN3/2019] |
| Uncontrolled Keywords: | Alginate;Sargassum ilicifolium; mangosteen rind;Wound healing;Diabetic mice |
| Subjects: | Q Science > QC Physics Q Science > QD Chemistry R Medicine R Medicine > RC Internal medicine R Medicine > RC Internal medicine > RC1200 Sports Medicine |
| Divisions: | Faculty of Science |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 13 Sep 2022 07:18 |
| Last Modified: | 13 Sep 2022 07:18 |
| URI: | http://eprints.um.edu.my/id/eprint/34403 |
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