Inhibitory potential of Quercus lusitanica extract on dengue virus type 2 replication

Muliawan, S.Y.; Kit, L.S.; Devi, S.; Hashim, O.; Yusof, R. (2006) Inhibitory potential of Quercus lusitanica extract on dengue virus type 2 replication. Southeast Asian Journal of Tropical Medicine and Public Health, 37 (Suppl.). pp. 132-5.

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    Abstract

    This study reports the in vitro inhibitory potential of crude extract of Quercus lusitanica (Q. lusitanica) seeds on the replication of dengue virus type 2 (DEN-2). In vitro antiviral activity of Q. lusitanica extract, assessed in C6/36 cells (cloned cells of Aedes albopictus larvae) employing a virus inhibition assay, showed dose-dependent inhibition. The Q. lusitanica extract at its maximum non-toxic concentration of 0.25 mg/ml completely inhibited 10-1,000 TCID50 of virus, as indicated by the absence of cytopathic effect (CPE). The low dose of Q. lusitanica (0.032 mg/ml) showed 100 inhibition with 10 TCID50 of virus, but only 50 and 25 inhibition with 100 and 1,000 TCID50, respectively. The NS1 is a glycoprotein present in all flaviviruses and appears essential for virus viability. To further evaluate Q. lusitanica extract as an antiviral compound, we investigated the effect of Q. lusitanica extract on the NS1 protein expression of infected C6/36 cells through proteomics technique. The result showed downregulation of NS1 protein expression of infected C6/36 cells after treatment with this extract. In conclusion, we found that Q. lusitanica extract has a good inhibitory effect on the replication of dengue virus type 2, both in conventional cell culture and proteomics technique

    Item Type: Article
    Creators:
    1. Muliawan, S.Y.
    2. Kit, L.S.
    3. Devi, S.
    4. Hashim, O.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    5. Yusof, R.
    Journal or Publication Title: Southeast Asian Journal of Tropical Medicine and Public Health
    Additional Information: correspondence: Sylvia y muliawan, department of microbiology, faculty of medicine, trisakti university, Jakarta 11440, indonesia. tel: (62)(21)-5672731, -5655786; fax: (62)(21)-5660706 E-mail: sylviaym2003@yahoo.com
    Uncontrolled Keywords: Dengue virus
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 10 Jul 2012 10:23
    Last Modified: 10 Jul 2012 10:23
    URI: http://eprints.um.edu.my/id/eprint/3440

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