Expression of high-abundance proteins in sera of patients with endometrial and cervical cancers: analysis using 2-DE with silver staining and lectin detection methods

Abdul-Rahman, P.S.; Lim, B.K.; Hashim, O.H. (2007) Expression of high-abundance proteins in sera of patients with endometrial and cervical cancers: analysis using 2-DE with silver staining and lectin detection methods. Electrophoresis, 28 (12). pp. 1989-1996. ISSN 0173-0835

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    Abstract

    The expression of high-abundance serum proteins in newly diagnosed patients with endometrial adenocarcinoma (EACa), squarnous cell cervical carcinoma (SCCa) and cervical adenocarcinorna (ACCa), relative to control female subjects, was analyzed by subjecting serum samples to 2-DE followed by image analysis of the silver-stained protein profiles. The three cohorts of cancer patients demonstrated different altered expression of serum high-abundance proteins compared to negative control women. Tl-ie expression of alpha(1)-antitrypsin, alpha(1)-B glycoprotein, cleaved high-molecular-weight kininogen (light chain) and antithrombin III were consistently altered in all the patients. However, clusterin was upregulated only in the patients with EACa, while those with SCCa and ACCa were typically characterized by the upregulated expression of zinc alpha-2-glycoprotein. The aberrant expression of selective serum proteins in the various cohorts of cancer patients was validated by competitive ELISA as well as by lectin detection. Analysis by using the charnpedak galactose binding lectin further highlighted an unidentified protein that may be differently glycosylated in the sera of the EACa patients that were studied.

    Item Type: Article
    Creators:
    1. Abdul-Rahman, P.S.
    2. Lim, B.K.
    3. Hashim, O.H.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    Journal or Publication Title: Electrophoresis
    Additional Information: Abdul-Rahman, Puteri S. Lim, Boon-Kiong Hashim, Onn H.
    Uncontrolled Keywords: Cervical canceer; Endometrial cancer; Lectin; O-Glycans; Proteomics
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 10 Jul 2012 09:47
    Last Modified: 10 Jul 2012 09:47
    URI: http://eprints.um.edu.my/id/eprint/3438

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