Panya, Aussara and Songprakhon, Pucharee and Panwong, Suthida and Jantakee, Kanyaluck and Kaewkod, Thida and Tragoolpua, Yingmanee and Sawasdee, Nunghathai and Lee, Vannajan Sanghiran and Nimmanpipug, Piyarat and Yenchitsomanus, Pa-thai (2021) Cordycepin inhibits virus replication in dengue virus-infected vero cells. Molecules, 26 (11). ISSN 1420-3049, DOI https://doi.org/10.3390/molecules26113118.
Full text not available from this repository.Abstract
Dengue virus (DENV) infection causes mild to severe illness in humans that can lead to fatality in severe cases. Currently, no specific drug is available for the treatment of DENV infection. Thus, the development of an anti-DENV drug is urgently required. Cordycepin (3 `-deoxyadenosine), which is a major bioactive compound in Cordyceps (ascomycete) fungus that has been used for centuries in Chinese traditional medicine, was reported to exhibit antiviral activity. However, the anti-DENV activity of cordycepin is unknown. We hypothesized that cordycepin exerts anti-DENV activity and that, as an adenosine derivative, it inhibits DENV replication. To test this hypothesis, we investigated the anti-DENV activity of cordycepin in DENV-infected Vero cells. Cordycepin treatment significantly decreased DENV protein at a half-maximal effective concentration (EC50) of 26.94 mu M. Moreover, DENV RNA was dramatically decreased in cordycepin-treated Vero cells, indicating its effectiveness in inhibiting viral RNA replication. Via in silico molecular docking, the binding of cordycepin to DENV non-structural protein 5 (NS5), which is an important enzyme for RNA synthesis, at both the methyltransferase (MTase) and RNA-dependent RNA polymerase (RdRp) domains, was predicted. The results of this study demonstrate that cordycepin is able to inhibit DENV replication, which portends its potential as an anti-dengue therapy.
| Item Type: | Article |
|---|---|
| Funders: | Center of Excellence for Innovation in Analytical Science and Technology (I-ANALY-S-T), Chiang Mai University, Thailand Research Fund (TRF) [DPG6080002], Chiang Mai University [R000026614] |
| Uncontrolled Keywords: | Bioactive compound; Cordycepin; Cordyceps extract; Dengue virus; Antiviral activity |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Department of Chemistry |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 09 Jun 2022 04:21 |
| Last Modified: | 09 Jun 2022 04:21 |
| URI: | http://eprints.um.edu.my/id/eprint/34378 |
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