Chiu, Hock Ing and Che Mood, Che Nurul Azieyan and Mohamad Zain, Nur Nadhirah and Ramachandran, Muggundha Raoov and Yahaya, Noorfatimah and Nik Mohamed Kamal, Nik Nur Syazni and Tung, Wai Hau and Yong, Yoke Keong and Lee, Chee Keong and Lim, Vuanghao (2021) Biogenic silver nanoparticles of clinacanthus nutans as antioxidant with antimicrobial and cytotoxic effects. Bioinorganic Chemistry and Applications, 2021. DOI https://doi.org/10.1155/2021/9920890.
Full text not available from this repository.Abstract
Silver nanoparticles (AgNPs) previously synthesised using leaf (AgNP-L) and stem (AgNP-S) extracts of Clinacanthus nutans (C. nutans) were tested to evaluate antimicrobial, antioxidant, and cytotoxicity activities. The AgNPs showed good inhibition against bacteria, but not fungi. The inhibition results showed the highest activity against Staphylococcus aureus (S. aureus) with 11.35 mm (AgNP-L) and 11.52 mm (AgNP-S), while the lowest inhibition was against Escherichia coli (E. coli) with 9.22 mm (AgNP-L) and 9.25 mm (AgNP-S) in the disc diffusion method. The same trend of results was noted in the well diffusion method. The IC50 of AgNP-L and AgNP-S in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2 `-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays was 417.05 mu g/mL and 434.60 mu g/mL, as well as 304.31 mu g/mL and 326.83 mu g/mL, respectively. Ferric reducing power (FRAP) assay showed that AgNP-L 872.389 mu mol/L Fe(II)] and AgNP-S 612.770 mu mol/L Fe(II)] exhibited significantly (p < 0.05) greater antioxidant activities than leaf extract (CNL) 152.260 mu mol/L Fe(II)] and stem extract (CNS) 110.445 mu mol/L Fe(II)] of C. nutans. The AgNPs were also proven to possess cytotoxic effects on the breast (MCF-7), cervical (HeLa), and colon (HT-29) cancer cells in a dose-dependent manner. AgNP-S and AgNP-L showed significantly (p < 0.05) higher cytotoxicity against MCF-7 (117.43 mu g/mL) and HT-29 (78.47 mu g/mL), respectively. In conclusion, the biosynthesised AgNPs from aqueous extract leaves and stem of C. nutans have demonstrated promising potential towards antioxidant, antimicrobial, and cytotoxicity activities.
| Item Type: | Article |
|---|---|
| Funders: | Fundamental Research Grant Scheme (FRGS)[FRGS/1/2018/STG07/USM/02/9 ], Ministry of Education, Malaysia[203.CIPPT.6711684] |
| Uncontrolled Keywords: | Cellular-responses;Escherichia-coli;Oxidative stress;Green synthesis;Leaf extract;Antibacterial;Biosynthesis |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Faculty of Science |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 15 Jun 2022 07:38 |
| Last Modified: | 15 Jun 2022 07:38 |
| URI: | http://eprints.um.edu.my/id/eprint/34335 |
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