Identification of immunogenic proteins from Burkholderia cepacia secretome using proteomic analysis

Mariappan, V.; Vellasamy, K.M.; Thimma, J.S.; Hashim, O.H.; Vadivelu, J. (2010) Identification of immunogenic proteins from Burkholderia cepacia secretome using proteomic analysis. Vaccine, 28 (5). pp. 1318-1324. ISSN 0264-410X

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    Abstract

    Burkholderia cepacia is an opportunistic human pathogen associated with lung infections. Secretory proteins of B. cepacia are known to be involved in virulence and may mediate important host-pathogen interactions. In the present study, secretory proteins isolated from B. cepacia culture supernatant were separated using two-dimensional gel electrophoresis, followed by Western blot analysis to identify the immunogenic proteins. Mice antibodies raised to B. cepacia inactivated whole bacteria, outer membrane protein and culture filtrate antigen detected 74,104 and 32 immunogenic proteins, respectively. Eighteen of these immunogenic proteins which reacted with all three antibodies were identified and might be potential molecules as a diagnostic marker or a putative candidate vaccine against B. cepacia infections. (C) 2009 Elsevier Ltd. All rights reserved.

    Item Type: Article
    Creators:
    1. Mariappan, V.
    2. Vellasamy, K.M.
    3. Thimma, J.S.
    4. Hashim, O.H.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    5. Vadivelu, J.
    Journal or Publication Title: Vaccine
    Additional Information: ∗ Corresponding author. Tel.: +60 3 7967 6662; fax: +60 3 7967 6671. E-mail address: jamuna@um.edu.my (J. Vadivelu).
    Uncontrolled Keywords: Burkholderia cepacia; Secretome; Proteomic; Immunogenic
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 10 Jul 2012 09:11
    Last Modified: 10 Jul 2012 09:11
    URI: http://eprints.um.edu.my/id/eprint/3433

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