Banavar, Spoorthi Ravi and Rawal, Swati Yeshwant and Pulikkotil, Shaju Jacob and Daood, Umer and Paterson, Ian C. and Davamani, Fabian Amalraj and Kajiya, Mikihito and Kurihara, Hidemi and Khoo, Suan Phaik and Tan, Eng Lai (2021) 3D clumps/extracellular matrix complexes of periodontal ligament stem cells ameliorate the attenuating effects of LPS on proliferation and osteogenic potential. Journal of Personalized Medicine, 11 (6). ISSN 2075-4426, DOI https://doi.org/10.3390/jpm11060528.
Full text not available from this repository.Abstract
The effects of lipopolysaccharide (LPS) on cell proliferation and osteogenic potential (OP) of MSCs have been frequently studied. Objective: to compare the effects of LPS on periodontal-ligament-derived mesenchymal stem cells (PDLSCs) in monolayer and 3D culture. Methods: The PDLSCs were colorimetrically assessed for proliferation and osteogenic potential (OP) after LPS treatment. The 3D cells were manually prepared by scratching and allowing them to clump up. The clumps (C-MSCs) were treated with LPS and assessed for Adenosine triphosphate (ATP) and OP. Raman spectroscopy was used to analyze calcium salts, DNA, and proline/hydroxyproline. Multiplexed ELISA was performed to assess LPS induced local inflammation. Results: The proliferation of PDLSCs decreased with LPS. On Day 28, LPS-treated cells showed a reduction in their OP. C-MSCs with LPS did not show a decrease in ATP production. Principal bands identified in Raman analysis were the P-O bond at 960 cm(-1) of the mineral component, 785 cm(-1), and 855 cm(-1) showing qualitative changes in OP, proliferation, and proline/hydroxyproline content, respectively. ELISA confirmed increased levels of IL-6 and IL-8 but with the absence of TNF-alpha and IL-1 beta secretion. Conclusions: These observations demonstrate that C-MSCs are more resistant to the effects of LPS than cells in monolayer cell culture. Though LPS stimulation of C-MSCs creates an early pro-inflammatory milieu by secreting IL-6 and IL-8, PDLSCs possess inactivated TNF promoter and an ineffective caspase-1 activating process.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education (MoHE) under the Fundamental Research Grant Scheme (FRGS)[FRGS/1/2016/SKK14/IMU/01/1], Ministry of Higher Education (MoHE) under the Fundamental Research Grant Scheme (FRGS)[ID-10093], Ministry of Higher Education (MoHE) under the Fundamental Research Grant Scheme (FRGS)[FRGS 2016-1] |
| Uncontrolled Keywords: | Stem cells;Mesenchymal stem cells;Periodontal stem cells;3D cells;Organoids;Periodontitis;Regeneration;Osteogenic potential; lipopolysaccharide; proliferation; Alizarin-Red; cetylpyridinium chloride; cytokines |
| Subjects: | R Medicine R Medicine > RC Internal medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 15 Jun 2022 07:55 |
| Last Modified: | 15 Jun 2022 07:55 |
| URI: | http://eprints.um.edu.my/id/eprint/34325 |
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