Application of champedak mannose-binding lectin in the glycoproteomic profiling of serum samples unmasks reduced expression of alpha-2 macroglobulin and complement factor B in patients with nasopharyngeal carcinoma

Seriramalu, R.; Pang, W.W.; Jayapalan, J.J.; Mohamed, E.; Abdul-Rahman, P.S.; Bustam, A.Z.; Khoo, A.S.B.; Hashim, O.H. (2010) Application of champedak mannose-binding lectin in the glycoproteomic profiling of serum samples unmasks reduced expression of alpha-2 macroglobulin and complement factor B in patients with nasopharyngeal carcinoma. Electrophoresis, 31 (14). pp. 2388-2395. ISSN 0173-0835

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    Abstract

    The use of lectin affinity chromatography prior to 2-DE separation forms an alternative method to unmask the expression of targeted glycoproteins of lower abundance in serum samples. Reduced expression of alpha-2 macroglobulin (AMG) and complement factor B (CFB) was detected in sera of patients with nasopharyngeal carcinoma (NPC) when pooled serum samples of the patients and those of healthy individuals were subjected to affinity isolation using immobilized champedak mannose-binding lectin and analyzed by 2-DE and densitometry. The AMG and CFB spots were not detected in the 2-DE protein profiles when the same pooled serum samples were subjected to albumin and IgG depletion and neither were they detected when the depleted samples were analyzed by western blotting and lectin detection. Together with other acute-phase response proteins that were previously reported to be altered in expression in NPC patients, AMG and CFB may serve as useful complementary biomarkers for NPC.

    Item Type: Article
    Creators:
    1. Seriramalu, R.
    2. Pang, W.W.
    3. Jayapalan, J.J.
    4. Mohamed, E.
    5. Abdul-Rahman, P.S.
    6. Bustam, A.Z.
    7. Khoo, A.S.B.
    8. Hashim, O.H.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    Journal or Publication Title: Electrophoresis
    Additional Information: Correspondence: Professor Onn H. Hashim, Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: onnhashim@um.edu.my Fax: +603-79674957
    Uncontrolled Keywords: α-2 Macroglobulin; Biomarker; Complement factor B; Lectin; Nasopharyngeal carcinoma
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 09 Jul 2012 16:50
    Last Modified: 09 Jul 2012 16:50
    URI: http://eprints.um.edu.my/id/eprint/3431

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