Application of champedak mannose-binding lectin in the glycoproteomic profiling of serum samples unmasks reduced expression of alpha-2 macroglobulin and complement factor B in patients with nasopharyngeal carcinoma

Seriramalu, R. and Pang, W.W. and Jayapalan, J.J. and Mohamed, E. and Abdul-Rahman, P.S. and Bustam, A.Z. and Khoo, A.S.B. and Hashim, Onn Haji (2010) Application of champedak mannose-binding lectin in the glycoproteomic profiling of serum samples unmasks reduced expression of alpha-2 macroglobulin and complement factor B in patients with nasopharyngeal carcinoma. Electrophoresis, 31 (14). pp. 2388-2395. ISSN 0173-0835, DOI https://doi.org/10.1002/elps.201000164.

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Abstract

The use of lectin affinity chromatography prior to 2-DE separation forms an alternative method to unmask the expression of targeted glycoproteins of lower abundance in serum samples. Reduced expression of alpha-2 macroglobulin (AMG) and complement factor B (CFB) was detected in sera of patients with nasopharyngeal carcinoma (NPC) when pooled serum samples of the patients and those of healthy individuals were subjected to affinity isolation using immobilized champedak mannose-binding lectin and analyzed by 2-DE and densitometry. The AMG and CFB spots were not detected in the 2-DE protein profiles when the same pooled serum samples were subjected to albumin and IgG depletion and neither were they detected when the depleted samples were analyzed by western blotting and lectin detection. Together with other acute-phase response proteins that were previously reported to be altered in expression in NPC patients, AMG and CFB may serve as useful complementary biomarkers for NPC.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Correspondence: Professor Onn H. Hashim, Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: onnhashim@um.edu.my Fax: +603-79674957
Uncontrolled Keywords:	α-2 Macroglobulin; Biomarker; Complement factor B; Lectin; Nasopharyngeal carcinoma
Subjects:	R Medicine
Divisions:	Faculty of Medicine
Depositing User:	Ms Haslinda Lahuddin
Date Deposited:	09 Jul 2012 08:50
Last Modified:	24 Oct 2019 08:24
URI:	http://eprints.um.edu.my/id/eprint/3431

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