Identification of immunoreactive secretory proteins from the stationary phase culture of Burkholderia pseudomallei

Vellasamy, K.M.; Mariappan, V.; Hashim, O.H.; Vadivelu, J. (2011) Identification of immunoreactive secretory proteins from the stationary phase culture of Burkholderia pseudomallei. Electrophoresis, 32 (2). pp. 310-320. ISSN 0173-0835

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    Abstract

    Bacterial secreted proteins are known to be involved in virulence and may mediate important host-pathogen interactions. In this study, when the stationary phase culture supernatant of Burkholderia pseudomallei was subjected to 2-DE, 113 protein spots were detected. Fifty-four of the secreted proteins, which included metabolic enzymes, transcription/translation regulators, potential virulence factors, chaperones, transport regulators, and hypothetical proteins, were identified using MS and database search. Twelve of these proteins were apparently reactive to antisera of mice that were immunised with B. pseudomallei secreted proteins. These proteins might be excellent candidates to be used as diagnostic markers or putative candidate vaccines against B. pseudomallei infections.

    Item Type: Article
    Creators:
    1. Vellasamy, K.M.
    2. Mariappan, V.
    3. Hashim, O.H.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    4. Vadivelu, J.
    Journal or Publication Title: Electrophoresis
    Additional Information: Correspondence: Professor Jamuna Vadivelu, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: jamuna@um.edu.my Fax: 01603-7967-6664
    Uncontrolled Keywords: Burkholderia pseudomallei; Immunoreactive; Proteomics; Secreted proteins
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 09 Jul 2012 16:44
    Last Modified: 09 Jul 2012 16:44
    URI: http://eprints.um.edu.my/id/eprint/3428

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