Wong, C.S.; Yin, W.F.; Sam, C.K.; Koh, C.L.; Chan, K.G. (2012) Characterization of wetland quorum quenching Pseudomonas aeruginosa strain 2SW8 and its 2-heptyl-3-hydroxy-4-quinolone production. The new microbiologica, 35 (1). ISSN 1121-7138Full text not available from this repository.
Most Proteobacteria produce N-acylhomoserine lactones for bacterial cell-to-cell communication, a process called quorum sensing. Interference of quorum sensing, commonly known as quorum quenching, represents an important way to control quorum sensing. This work reports the isolation of quorum quenching bacterium strain 2WS8 from Malaysia tropical wetland water (2°11'8"N, 102°15'2"E, in 2007) by using a modified version of a previously reported KG medium. Strain 2WS8 was isolated based on its ability to utilize N-(3-oxohexanoyl)-L-homoserine lactone (3-oxo-C6-HSL) as the sole source of energy. This bacterium clustered closely to Pseudomonas aeruginosa PAO1. Strain 2SW8 possesses both quiP and pvdQ homologue acylase genes. Rapid Resolution Liquid Chromatography analysis confirmed that strain 2SW8 preferentially degraded N-acylhomoserine lactones with 3-oxo group substitution but not those with unsubstituted groups at C3 position in the acyl side chain. Strain 2SW8 also showed 2-heptyl-3-hydroxy-4-quinolone production.
|Journal or Publication Title:||The new microbiologica|
|Additional Information:||Division of Genetics and Molecular Biology, University of Malaya, Kuala Lumpur, Malaysia|
|Uncontrolled Keywords:||Genetics and Molecular Biology|
|Divisions:||Faculty of Medicine|
|Depositing User:||Mr. Faizal Hamzah|
|Date Deposited:||02 Jul 2012 10:15|
|Last Modified:||02 Jul 2012 10:15|
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