Jones, Sherry Usun and Chua, Kek Heng and Chew, Ching Hoong and Yeo, Chew Chieng and Abdullah, Fatimah Haslina and Othman, Norlela and Kee, Boon Pin and Puah, Suat Moi (2021) Spa diversity of methicillin-resistant and -susceptible Staphylococcus aureus in clinical strains from Malaysia: A high prevalence of invasive European spa-type t032. PeerJ, 9. ISSN 2167-8359, DOI https://doi.org/10.7717/peerj.11195.
Full text not available from this repository.Abstract
Background. Staphylococcus aureus is one of the important pathogens causing nosocomial infection. spa typing allows identification of S. aureus clones in hospital isolates and is useful for epidemiological studies and nosocomial infection control. This study aims to investigate the spa types in Malaysian S. aureus isolates obtained from various clinical specimens. Method. A total of 89 methicillin-resistant S. aureus (MRSA) pus (n = 55), blood (n = 27), respiratory (n = 5), eye (n = 2)] isolates and 109 methicillin-susceptible S. aureus (MSSA) pus (n = 79), blood (n = 24), respiratory (n = 3), eye (n = 2) and urine (n = 1)] isolates were subjected to spa typing with sequences analysed using BioNumerics version 7. Results. The spa sequence was successfully amplified from 77.8% of the strains (154/198) and 47 known spa types were detected. The distribution of known spa types in MRSA (36.2%, 17/47) was less diverse than in MSSA (70.2%, 33/47). The most predominant spa types were t032 (50%) in MRSA, and t127 (19%) and t091 (16.7%) in MSSA, respectively. spa type t091 in MSSA was significantly associated with skin and soft tissue infections (p=0.0199). Conclusion. The previously uncommon spa type t032 was detected in the Malaysian MRSA strains, which also corresponded to the most common spa type in Europe and Australia, and has replaced the dominant spa type t037 which was reported in Malaysia in 2010.
| Item Type: | Article |
|---|---|
| Funders: | Fundamental Research Grant Scheme from the Malaysian Ministry of Higher Education[FRGS/1/2019/SKK08/UM/02/5] |
| Uncontrolled Keywords: | Methicillin-resistant Staphylococcus aureus;Methicillin-susceptible Staphylococcus aureus;Spa typing |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 18 Jul 2022 07:49 |
| Last Modified: | 18 Jul 2022 07:49 |
| URI: | http://eprints.um.edu.my/id/eprint/34094 |
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