Application of amplified ribosomal DNA restriction analysis in identification of Acinetobacter baumannii from a tertiary teaching hospital, Malaysia

Kong, B.H. and Hanifah, Y.A. and Yusof, M.Y. and Thong, Kwai Lin (2011) Application of amplified ribosomal DNA restriction analysis in identification of Acinetobacter baumannii from a tertiary teaching hospital, Malaysia. Tropical Biomedicine, 28 (3). pp. 563-568. ISSN 0127-5720

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Official URL: http://www.msptm.org/files/563_-_568_Thong_K_L.pdf

Abstract

Acinetobacter baumannii, genomic species 3 and 13TU are being increasingly reported as the most important Acinetobacter species that cause infections in hospitalized patients. These Acinetobacter species are grouped in the Acinetobacter calcoaceticus- Acinetobacter baumannii (Acb) complex. Differentiation of the species in the Acb-complex is limited by phenotypic methods. Therefore, in this study, amplified ribosomal DNA restriction analysis (ARDRA) was applied to confirm the identity A. baumannii strains as well as to differentiate between the subspecies. One hundred and eighty-five strains from Intensive Care Unit, Universiti Malaya Medical Center (UMMC) were successfully identified as A. baumannii by ARDRA. Acinetobacter genomic species 13TU and 15TU were identified in 3 and 1 strains, respectively. ARDRA provides an accurate, rapid and definitive approach towards the identification of the species level in the genus Acinetobacter. This paper reports the first application ARDRA in genospecies identification of Acinetobacter in Malaysia

Item Type: Article
Additional Information: Microbiology Division, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
Uncontrolled Keywords: Amplified ribosomal DNA restriction analysis; Acinetobacter baumannii; Tertiary teaching hospital; Malaysia
Subjects: R Medicine
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Mr. Faizal Hamzah
Date Deposited: 29 Jun 2012 08:19
Last Modified: 16 Oct 2018 03:37
URI: http://eprints.um.edu.my/id/eprint/3403

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