Reduced susceptibility of Malaysian clinical isolates of Burkholderia pseudomallei to ciprofloxacin

Liew , F.Y.; Tay , S.T.; Puthucheary , S.D. (2012) Reduced susceptibility of Malaysian clinical isolates of Burkholderia pseudomallei to ciprofloxacin. Tropical Biomedicine, 28 (3). ISSN 0127-5720

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Abstract

Ciprofloxacin, a quinolone with good intracellular penetration may possibly be used for treatment of melioidosis caused by Burkholderia pseudomallei, but problems with resistance may be encountered. Amino acid substitutions in gyrA/gyrB have given rise to fluoroquinolone resistance in various microorganisms. Using published primers for gyrA and gyrB, PCR was performed on 11 isolates of B. pseudomallei with varying degrees of sensitivity to ciprofloxacin, followed by DNA sequencing to detect possible mutations. Results showed an absence of any point mutation in either gene. Local isolates have yet to develop full resistance to ciprofloxacin and probably other mechanisms of resistance may have been involved in the decreased sensitivity to ciprofloxacin.

Item Type: Article
Journal or Publication Title: Tropical Biomedicine
Additional Information: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
Uncontrolled Keywords: Medical Microbiology
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Mr. Faizal Hamzah
Date Deposited: 29 Jun 2012 11:24
Last Modified: 29 Jun 2012 11:24
URI: http://eprints.um.edu.my/id/eprint/3402

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