Determination of the percentage inhibition of diameter growth (PIDG) of Piper betle crude aqueous extract against oral Candida species

Himratul-Aznita, W.H. and Mohd-Al-Faisal, N. and Fathilah, A.R. (2011) Determination of the percentage inhibition of diameter growth (PIDG) of Piper betle crude aqueous extract against oral Candida species. Journal of Medicinal Plants Research, 5 (6). pp. 878-884. ISSN 1996-0875


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Species within the genus Candida have been implicated in many fungal diseases such as candidiasis or thrush. The increasing clinical and microbiological resistance of Candida species towards several commonly prescribed antifungal agents however, has lead to the search for new active antifungal compounds from natural resources. This study was carried out to screen the susceptibility of the aqueous extract of Piper betle towards seven species of oral Candida. It was found that P. betle extract exhibited high antifungal activities towards Candida albicans, Candida tropicalis, Candida glabrata, Candida dubliniensis, Candida lusitaniae, Candida krusei and Candida parapsilosis. The minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) value of P. betle extract towards all Candida species was found to be similar (12 mg/ml) except towards C. albicans which has been shown to have MIC value of 12 mg/ml and slightly higher MFC value of 25 mg/ml. The recorded data on the growth responses of the species to various concentrations of the extract following a 24 h incubation period were analysed, using the percentage inhibition of diameter growth (PIDG) against chlorhexidine gluconate (CHX). The determination of PIDG values for C. albicans, C. tropicalis, C. lusitaniae, C. dubliniensis and C. glabrata has shown that the aqueous extract of P. betle outstrips the positive control used, that was 0.12 w/v chlorhexidine with PIDG values of more than 50 at P. betle concentration of 25 mg/ml. In contrast, PIDG for C. krusei and C. parapsilosis shows that at 25 mg/ml concentration of P. betle extract has little influence on growth inhibition compared to CHX. Thus, the results obtained have shown the potential use of P. betle extract as antifungal agent and thus significantly contribute to its antifungal development.

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Uncontrolled Keywords: Candida sp. oral Candida Piper betle antifungal activity percentage inhibition of diameter growth (PIDG) essential oil in-vitro antifungal agents periodontitis constituents infections membrane
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dept of Oral Biology
Depositing User: Mr Ahmad Azwan Azman
Date Deposited: 29 Jun 2012 02:03
Last Modified: 30 May 2017 12:25

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