Treatment outcomes of alginate-embedded allogenic mesenchymal stem cells versus autologous chondrocytes for the repair of focal articular cartilage defects in a rabbit model

Tay, L.X.; Ahmad, R.E.; Dashtdar, H.; Tay, K.W.; Masjuddin, T.; Ab-Rahim, S.; Chong, P.P.; Selvaratnam, L.; Kamarul, T. (2011) Treatment outcomes of alginate-embedded allogenic mesenchymal stem cells versus autologous chondrocytes for the repair of focal articular cartilage defects in a rabbit model. American Journal of Sports Medicine, 40 (1). pp. 83-90. ISSN 0363-5465

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    Background: Mesenchymal stem cells (MSCs) represent a promising alternative form of cell-based therapy for cartilage injury. However, the capacity of MSCs for chondrogenesis has not been fully explored. In particular, there is presently a lack of studies comparing the effectiveness of MSCs to conventional autologous chondrocyte (autoC) treatment for regeneration of full-thickness cartilage defects in vivo. Hypothesis: Treatment with allogenic undifferentiated MSCs (alloMSCs) results in superior cartilage tissue regeneration profiles when compared with autoC for repair of focal articular cartilage defects. Study Design: Controlled laboratory study. Methods: Full-thickness articular cartilage defects were created on the weightbearing surface of the medial femoral condyles in both knees of New Zealand White rabbits (N = 30). Six weeks after the defect was induced, the right knee was treated with either alloMSCs (n = 12) or autoC (n = 18), while the left knee remained untreated (control). The rabbits were sacrificed at 6 months after treatment for assessment of cartilage tissue regeneration, which included the Brittberg morphologic score, histologic grading by O'Driscoll score, and quantitative analysis of glycosaminoglycans per total protein content. Results: Apart from significantly higher Brittberg scores in the alloMSC treatment group (8.8 +/- 0.8) versus the autoC treatment group (6.6 +/- 0.8) (P = .04), both treatments showed similar cartilage regenerative profiles. All outcome measures were significantly higher in the treatment groups compared with their respective controls (P < .05). Conclusion: AlloMSCs have similar effectiveness as autoC for repair of focal cartilage defects. Both treatments resulted in superior tissue regeneration compared with untreated defects. Clinical Relevance: The results have an implication of supporting the potential use of MSCs for cartilage repair after sports injuries or diseases, in view of similar efficacy but less patient morbidity and potential cost savings as compared with conventional autoC therapy.

    Item Type: Article
    1. Tay, L.X.
    2. Ahmad, R.E.
    3. Dashtdar, H.
    4. Tay, K.W.
    5. Masjuddin, T.
    6. Ab-Rahim, S.
    7. Chong, P.P.
    8. Selvaratnam, L.
    9. Kamarul, T.(Department of Orthopaedic Surgery, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    Journal or Publication Title: American Journal of Sports Medicine
    Additional Information: Tay, Liang Xin Ahmad, Raja Elina Dashtdar, Havva Tay, K. W. Masjuddin, T. Ab-Rahim, S. Chong, Pan Pan Selvaratnam, L. Kamarul, T.
    Uncontrolled Keywords: Mesenchymal Stem Cell; Chondrogenic Differentiation; Cartilage Repair; Chondrocytes
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 29 Jun 2012 10:02
    Last Modified: 10 Dec 2013 13:13

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