Production of gymnemic acid depends on medium, explants, pgrs, color lights, temperature, photoperiod, and sucrose sources in batch culture of gymnema sylvestre

Ahmed, A.B.; Rao, A.S.; Rao, M.V.; Taha, R.M. (2012) Production of gymnemic acid depends on medium, explants, pgrs, color lights, temperature, photoperiod, and sucrose sources in batch culture of gymnema sylvestre. ScientificWorld Journal. ISSN 1537-744X

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    Abstract

    Gymnema sylvestre (R.Br.) is an important diabetic medicinal plant which yields pharmaceutically active compounds called gymnemic acid (GA). The present study describes callus induction and the subsequent batch culture optimization and GA quantification determined by linearity, precision, accuracy, and recovery. Best callus induction of GA was noticed in MS medium combined with 2,4-D (1.5 mg/L) and KN (0.5 mg/L). Evaluation and isolation of GA from the calluses derived from different plant parts, namely, leaf, stem and petioles have been done in the present case for the first time. Factors such as light, temperature, sucrose, and photoperiod were studied to observe their effect on GA production. Temperature conditions completely inhibited GA production. Out of the different sucrose concentrations tested, the highest yield (35.4 mg/g d.w) was found at 5% sucrose followed by 12 h photoperiod (26.86 mg/g d.w). Maximum GA production (58.28 mg/g d.w) was observed in blue light. The results showed that physical and chemical factors greatly influence the production of GA in callus cultures of G. sylvestre. The factors optimized for in vitro production of GA during the present study can successfully be employed for their large-scale production in bioreactors.

    Item Type: Article
    Journal or Publication Title: ScientificWorld Journal
    Additional Information: Correspondence should be addressed to A. Bakrudeen Ali Ahmed, dr.bakrudeenaliahmed@yahoo.co.in
    Uncontrolled Keywords: Biological Sciences
    Subjects: R Medicine
    Divisions: Faculty of Science
    Depositing User: Mr. Faizal Hamzah
    Date Deposited: 22 Jun 2012 09:58
    Last Modified: 11 Nov 2013 11:10
    URI: http://eprints.um.edu.my/id/eprint/3391

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