Krishnan, T.; Yin, W.F.; Chan, K.G. (2012) Inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) bud extract. Sensors (Basel)., 12 (4).
Quorum sensing controls the virulence determinants in most proteobacteria. In this work, the hexane, chloroform and methanol extracts of an Ayurveda spice, namely clove (Syzygium aromaticum), shown anti-quorum sensing activity. Hexane and methanol extracts of clove inhibited the response of C. violaceum CV026 to exogenously supplied N-hexanoylhomoserine lactone, in turn preventing violacein production. Chloroform and methanol extracts of clove significantly reduced bioluminescence production by E. coli [pSB1075] grown in the presence of N-(3-oxododecanoyl)-l-homoserine lactone. We demonstrated that clove extract inhibited quorum sensing-regulated phenotypes in Pseudomonas aeruginosa PA01, including expression of lecA::lux (by hexane extract), swarming (maximum inhibition by methanol extract), pyocyanin (maximum inhibition by hexane extract). This study shows that the presence of natural compounds that exhibit anti-quorum sensing activity in the clove extracts may be useful as the lead of anti-infective drugs.
|Item Type: ||Article|
- Krishnan, T.
- Yin, W.F.
- Chan, K.G.
|Journal or Publication Title: ||Sensors (Basel).|
|Additional Information: ||Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia; E-Mails: firstname.lastname@example.org (T.K.); email@example.com (W.F.Y.).|
|Uncontrolled Keywords: ||Ayurveda; bioluminescence; biosensors; Chromobacterium violaceum; lecA::lux; N-acylhomoserine lactones; Pseudomonas aeruginosa; pyocyanin; Syzygium aromaticum; swarming|
|Subjects: ||R Medicine|
|Divisions: ||Faculty of Medicine|
|Depositing User: ||Mr. Faizal Hamzah|
|Date Deposited: ||21 Jun 2012 09:29|
|Last Modified: ||11 Dec 2013 14:44|
Actions (For repository staff only: Login required)