Inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) bud extract

Krishnan, T.; Yin, W.F.; Chan, K.G. (2012) Inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) bud extract. Sensors (Basel)., 12 (4).

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    Abstract

    Quorum sensing controls the virulence determinants in most proteobacteria. In this work, the hexane, chloroform and methanol extracts of an Ayurveda spice, namely clove (Syzygium aromaticum), shown anti-quorum sensing activity. Hexane and methanol extracts of clove inhibited the response of C. violaceum CV026 to exogenously supplied N-hexanoylhomoserine lactone, in turn preventing violacein production. Chloroform and methanol extracts of clove significantly reduced bioluminescence production by E. coli [pSB1075] grown in the presence of N-(3-oxododecanoyl)-l-homoserine lactone. We demonstrated that clove extract inhibited quorum sensing-regulated phenotypes in Pseudomonas aeruginosa PA01, including expression of lecA::lux (by hexane extract), swarming (maximum inhibition by methanol extract), pyocyanin (maximum inhibition by hexane extract). This study shows that the presence of natural compounds that exhibit anti-quorum sensing activity in the clove extracts may be useful as the lead of anti-infective drugs.

    Item Type: Article
    Creators:
    1. Krishnan, T.
    2. Yin, W.F.
    3. Chan, K.G.
    Journal or Publication Title: Sensors (Basel).
    Additional Information: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia; E-Mails: thibavengdes@yahoo.com (T.K.); yinwaifong@yahoo.com (W.F.Y.).
    Uncontrolled Keywords: Ayurveda; bioluminescence; biosensors; Chromobacterium violaceum; lecA::lux; N-acylhomoserine lactones; Pseudomonas aeruginosa; pyocyanin; Syzygium aromaticum; swarming
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Mr. Faizal Hamzah
    Date Deposited: 21 Jun 2012 09:29
    Last Modified: 11 Dec 2013 14:44
    URI: http://eprints.um.edu.my/id/eprint/3377

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