Screening and effect evaluation of chemical inducers for enhancing astaxanthin and lipid production in mixotrophic Chromochloris zofingiensis

Chen, Jun-Hui and Wei, Dong and Lim, Phaik-Eem and Xie, Jun and Chen, Wei Ning (2022) Screening and effect evaluation of chemical inducers for enhancing astaxanthin and lipid production in mixotrophic Chromochloris zofingiensis. Journal of Applied Phycology, 34 (1). pp. 159-176. ISSN 0921-8971, DOI https://doi.org/10.1007/s10811-021-02618-6.

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Abstract

A two-stage screening approach of chemical inducers was carried out to evaluate the positive effect on enhancing astaxanthin and lipid production in mixotrophic Chromochloris zofingiensis. The results demonstrated that oxidants were able to increase the astaxanthin content high up to 11.4 mg g(-1) biomass, while pyruvic acid promoted the cell growth and increased the contents of astaxanthin and lipids up to 10.7 mg g(-1) and 66.1% dry weight. The highest yield (87.0 mg L-1) and productivity (7.3 mg L-1 day(-1)) of astaxanthin were obtained in parallel with high lipid productivity (459.0 mg L-1 day(-1)). The correlations between the dosage effect of chemical inducers and metabolites variation were further examined by statistical analyses. Additionally, C. zofingiensis-derived lipids were estimated to be more viable and feasible as edible oils based on fatty acid characteristics assessment. The present work highlights the effective screening strategy of chemical inducers for enhancing astaxanthin and lipid production and the potential application in mixotrophic C. zofingiensis.

Item Type: Article
Funders: Guangdong Basic and Applied Basic Research Foundation[2019A1515110591], Guangdong Basic and Applied Basic Research Foundation[2019B1515120002], National Key Research and Development Project[2019YFD0900302]
Uncontrolled Keywords: Chromochloris zofingiensis;Astaxanthin;Lipid;Chemical inducer;Regulatory mechanism
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Divisions: Faculty of Science
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 22 Jul 2022 08:21
Last Modified: 22 Jul 2022 08:21
URI: http://eprints.um.edu.my/id/eprint/33725

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