Lee, Wenn-Chyau and Shahari, Shahhaziq and Nguee, Samantha Yee Teng and Lau, Yee-Ling and Renia, Laurent (2022) Cytoadherence properties of plasmodium knowlesi-infected erythrocytes. Frontiers in Microbiology, 12. ISSN 1664-302X, DOI https://doi.org/10.3389/fmicb.2021.804417.
Full text not available from this repository.Abstract
Plasmodium knowlesi is responsible for zoonotic malaria infections that are potentially fatal. While the severe pathology of falciparum malaria is associated with cytoadherence phenomena by Plasmodium falciparum-infected erythrocytes (IRBC), information regarding cytoadherence properties of P. knowlesi-IRBC remained scarce. Here, we characterized the cytoadherence properties of RBC infected with the laboratory-adapted P. knowlesi A1-H.1 strain. We found that late-stage IRBC formed rosettes in a human serum-dependent manner, and rosettes hampered IRBC phagocytosis. IRBC did not adhere much to unexposed (unstimulated) human endothelial cell lines derived from the brain (hCMEC/D3), lungs (HPMEC), and kidneys (HRGEC). However, after being ``primed'' with P. knowlesi culture supernatant, the IRBC-endothelial cytoadherence rate increased in HPMEC and HRGEC, but not in hCMEC/D3 cells. Both endothelial cytoadherence and rosetting phenomena were abrogated by treatment of P. knowlesi-IRBC with trypsin. We also found that different receptors were involved in IRBC cytoadherence to different types of endothelial cells. Although some of the host receptors were shared by both P. falciparum- and P. knowlesi-IRBC, the availability of glycoconjugates on the receptors might influence the capacity of P. knowlesi-IRBC to cytoadhere to these receptors.
| Item Type: | Article |
|---|---|
| Funders: | Agency for Science Technology & Research (ASTAR), Open Fund-Young Individual Research Grant [Grant No: OF-YIRG NMRC/OFYIRG/0070/2018], Agency for Science Technology & Research (ASTAR) [Grant No: JCO-DP BMSI/15-800006-SIGN], Singapore Ministry of Education AcRF Tier 3 grant [Grant No: MOE2019-T3-1-007], Yong Loo Lin School of Medicine, NUS |
| Uncontrolled Keywords: | Plasmodium knowlesi; Cytoadherence; Endothelial; Rosette; Receptors |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 29 Jul 2022 03:10 |
| Last Modified: | 29 Jul 2022 03:10 |
| URI: | http://eprints.um.edu.my/id/eprint/33500 |
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