High diversity of cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene

Iqbal, A.; Lim, Y.A.L.; Surin, J.; Sim, B L.H. (2012) High diversity of cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene. Plos One, 7 (2). ISSN 1932-6203

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    Abstract

    Background: Currently, there is a lack of vital information in the genetic makeup of Cryptosporidium especially in developing countries. The present study aimed at determining the genotypes and subgenotypes of Cryptosporidium in hospitalized Malaysian human immunodeficiency virus (HIV) positive patients. Methodology/Principal Findings: In this study, 346 faecal samples collected from Malaysian HIV positive patients were genetically analysed via PCR targeting the 60 kDa glycoprotein (gp60) gene. Eighteen (5.2 of 346) isolates were determined as Cryptosporidium positive with 72.2 (of 18) identified as Cryptosporidium parvum whilst 27.7 as Cryptosporidium hominis. Further gp60 analysis revealed C. parvum belonging to subgenotypes IIaA13G1R1 (2 isolates), IIaA13G2R1 (2 isolates), IIaA14G2R1 (3 isolates), IIaA15G2R1 (5 isolates) and IIdA15G1R1 (1 isolate). C. hominis was represented by subgenotypes IaA14R1 (2 isolates), IaA18R1 (1 isolate) and IbA10G2R2 (2 isolates). Conclusions/Significance: These findings highlighted the presence of high diversity of Cryptosporidium subgenotypes among Malaysian HIV infected individuals. The predominance of the C. parvum subgenotypes signified the possibility of zoonotic as well as anthroponotic transmissions of cryptosporidiosis in HIV infected individuals.

    Item Type: Article
    Creators:
    1. Iqbal, A.
    2. Lim, Y.A.L.
    3. Surin, J.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    4. Sim, B L.H.
    Journal or Publication Title: Plos One
    Additional Information: Citation: Iqbal A, Lim YAL, Surin J, Sim BLH (2012) High Diversity of Cryptosporidium Subgenotypes Identified in Malaysian HIV/AIDS Individuals Targeting gp60 Gene. PLoS ONE 7(2): e31139. doi:10.1371/journal.pone.0031139
    Uncontrolled Keywords: HIV/AIDS
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: jsurin johari surin
    Date Deposited: 19 Jun 2012 12:06
    Last Modified: 19 Jun 2012 12:06
    URI: http://eprints.um.edu.my/id/eprint/3339

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