Ali, Hanisah and Sallahuddin, Nurhanani and Ahmed Shamsudin, Nur Haziqah and Mohd Zain, Nurul Amalina and Ibrahim, Mohd Hafiz and Yaacob, Jamilah Syafawati (2022) Abiotic stress induces morphological, physiological, and genetic changes in orthosiphon stamineus benth. in vitro cultures. Horticulturae, 8 (2). ISSN 2311-7524, DOI https://doi.org/10.3390/horticulturae8020153.
Full text not available from this repository.Abstract
Orthosiphon stamineus is a well-known medicinal herb that has long been used as a traditional treatment for a variety of ailments and disorders. In this study, in vitro grown O. stamineus plantlets were subjected to various stress factors to elucidate the effects of these stresses on the occurrence of somaclonal variation in this species. Murashige and Skoog (MS) media supplemented with 2.0 mg/L kinetin plus 0.5 mg/L IAA was identified as the optimum in vitro regeneration media (OM). The data analysis confirmed the clonal nature of the resulting plantlets. The plantlets were also exposed to various stress factors such as high salinity, abscisic acid (ABA), and high cytokinin concentrations, by subculturing the plantlets on OM added with various concentrations of NaCl, ABA, and high kinetin levels. Exposure to the various stress factors was observed to result in the occurrence of somaclonal variation in O. stamineus, where the incidence of hyperhydricity was found to be the most prominent. An ISSR analysis revealed 70.12% polymorphism among the variants. The results showed that the chlorophyll content and photosynthetic ability of plantlets were reduced when the abiotic stress levels increased.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya, Malaysia [RU004C-2020] [PG156-2014B] |
| Uncontrolled Keywords: | Orthosiphon stamineus; Plant tissue culture; ISSR; Hyperhydricity; Photosynthetic ability |
| Subjects: | S Agriculture > S Agriculture (General) S Agriculture > SB Plant culture |
| Divisions: | Faculty of Science > Institute of Biological Sciences Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 05 Aug 2022 00:08 |
| Last Modified: | 05 Aug 2022 00:08 |
| URI: | http://eprints.um.edu.my/id/eprint/33383 |
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