Lai , S.L.; Cheah , S.C.; Wong , P.F.; Noor , S.M.; Mustafa , M.R. (2012) In vitro and in vivo anti-angiogenic activities of panduratin a. PLoS One, 7 (5).
Abstract
BACKGROUND: Targeting angiogenesis has emerged as an attractive and promising strategy in anti-cancer therapeutic development. The present study investigates the anti-angiogenic potential of Panduratin A (PA), a natural chalcone isolated from Boesenbergia rotunda by using both in vitro and in vivo assays. METHODOLOGY/PRINCIPAL FINDINGS: PA exerted selective cytotoxicity on human umbilical vein endothelial cells (HUVECs) with IC(50) value of 6.91±0.85 µM when compared to human normal fibroblast and normal liver epithelial cells. Assessment of the growth kinetics by cell impedance-based Real-Time Cell Analyzer showed that PA induced both cytotoxic and cytostatic effects on HUVECs, depending on the concentration used. Results also showed that PA suppressed VEGF-induced survival and proliferation of HUVECs. Furthermore, endothelial cell migration, invasion, and morphogenesis or tube formation demonstrated significant time- and dose-dependent inhibition by PA. PA also suppressed matrix metalloproteinase-2 (MMP-2) secretion and attenuated its activation to intermediate and active MMP-2. In addition, PA suppressed F-actin stress fiber formation to prevent migration of the endothelial cells. More importantly, anti-angiogenic potential of PA was also evidenced in two in vivo models. PA inhibited neo-vessels formation in murine Matrigel plugs, and angiogenesis in zebrafish embryos. CONCLUSIONS/SIGNIFICANCE: Taken together, our study demonstrated the distinctive anti-angiogenic properties of PA, both in vitro and in vivo. This report thus reveals another biological activity of PA in addition to its reported anti-inflammatory and anti-cancer activities, suggestive of PA's potential for development as an anti-angiogenic agent for cancer therapy.
| Item Type: | Article |
|---|
| Creators: | - Lai , S.L.
- Cheah , S.C.
- Wong , P.F.
- Noor , S.M.
- Mustafa , M.R.
|
|---|
| Journal or Publication Title: | PLoS One |
|---|
| Additional Information: | Centre of Natural Products & Drug Discovery (CENAR), Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, and Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia |
|---|
| Uncontrolled Keywords: | Molecular Medicine; Pharmacology |
|---|
| Subjects: | R Medicine |
|---|
| Divisions: | UNSPECIFIED |
|---|
| Depositing User: | Mr. Faizal Hamzah |
|---|
| Date Deposited: | 15 Jun 2012 10:03 |
|---|
| Last Modified: | 15 Jun 2012 10:03 |
|---|
| URI: | http://eprints.um.edu.my/id/eprint/3313 |
|---|
Actions (login required)
![[img]](http://eprints.um.edu.my/style/images/fileicons/application_pdf.png)
