Cucurbitacin L 2-O-β-Glucoside demonstrates apoptogenesis in colon adenocarcinoma cells (HT-29): involvement of reactive oxygen and nitrogen species regulation

Abdelwahab, S.I. and Hassan, L.E. and Abdul Majid, A.M. and Yagi, S.M. and Mohan, S. and Elhassan Taha, M.M. and Ahmad, S. and Chuen, C.S. and Narrima, P. and Rais, M.M. and Syam, S. and Moharam, B.A. and Hadi, A.H. (2012) Cucurbitacin L 2-O-β-Glucoside demonstrates apoptogenesis in colon adenocarcinoma cells (HT-29): involvement of reactive oxygen and nitrogen species regulation. Evidence-Based Complementary and Alternative Medicine. ISSN 1741-427X

[img]
Preview
PDF
1.pdf

Download (2MB)
Official URL: http://www.ncbi.nlm.nih.gov/pubmed/22685485

Abstract

Emerging evidence suggests that reactive oxygen (ROS) and nitrogen (RNS) species can contribute to diverse signalling pathways of inflammatory and tumour cells. Cucurbitacins are a group of highly oxygenated triterpenes. Many plants used in folk medicine to treat cancer have been found to contain cucurbitacins displaying potentially important anti-inflammatory actions. The current study was designed to investigate the anti-ROS and -RNS effects of cucurbitacin L 2-O-β-glucoside (CLG) and the role of these signaling factors in the apoptogenic effects of CLG on human colon cancer cells (HT-29). This natural cucurbitacin was isolated purely from Citrullus lanatus var. citroides (Cucurbitaceae). The results revealed that CLG was cytotoxic to HT-29. CLG increased significantly (P < 0.05) RNA and protein levels of caspase-3 in HT-29 cells when verified using a colorimetric assay and realtime qPCR, respectively. The results showed that lipopolysaccharide/interferon-gamma (LPS/INF-γ) increased nitrous oxide (NO) production inR AW264.7macrophages, whereas N(G)-nitro-L-argininemethyl ester (L-NAME) and CLG curtailed it. This compound did not reveal any cytotoxicity on RAW264.7 macrophages and human normal liver cells (WRL-68) when tested using the MTT assay. Findings of ferric reducing antioxidant power (FRAP) and oxygen radical absorption capacity (ORAC) assays demonstrate the antioxidant properties of CLG. The apoptogenic property of CLG on HT-29 cells is thus related to inhibition of reactive nitrogen and oxygen reactive species and the triggering of caspase-3-regulated apoptosis.

Item Type: Article
Additional Information: Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
Uncontrolled Keywords: Pharmacy
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Medicine
Depositing User: Mr. Faizal Hamzah
Date Deposited: 15 Jun 2012 01:19
Last Modified: 27 Sep 2013 02:41
URI: http://eprints.um.edu.my/id/eprint/3309

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year