The effect of psidium guajava and piper betle extracts on the morphology of dental plaque bacteria

Razak, F.A.; Yusoff, M.; Rahim, Z.H.A. (2009) The effect of psidium guajava and piper betle extracts on the morphology of dental plaque bacteria. Pakistan Journal of Medical Sciences, 25 (6). pp. 928-933. ISSN 1682-024X

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    Abstract

    Objective: It has been reported that the aqueous extracts of Psidium guajava and Piper betle leaves showed anti-plaque activities during the early stages of dental plaque formation. The aim of the study was to elucidate if such anti-plaque activities involve any ultra-structural changes to the morphology of three early dental plaque bacteria, Streptococcus sanguinis, Streptococcus mitis and Actinomyces sp. Methodology: Pure cultures of the bacteria were suspended in BHI medium and treated with the test herbal extracts at the sub-lethal concentrations. The growth mixtures were incubated at 37 degrees C. At the logarithmic growth phase (t(1)), aliquots of 1 ml of the growth mixtures were fixed and used in the preparation of specimens for SEM studies. Ultra-structural alterations to the morphology of the treated cells noted were compared to those of the cells cultured under untreated conditions. Results: Following exposure of the bacteria to the two test herbal extracts, profound ultra-structural changes to their morphology were observed. The observed structural or morphological alterations could attribute to the bacteria being less active in performing normal physiological metabolic functions and thus rendering them less efficient to multiply. The changes noted included (i) reduced sizes of the bacteria, and (ii) majority cells at the non-dividing state as compared to those cultured under controlled conditions. Conclusions: This study has shown anti-plaque effects of aqueous extract of both P. betle and P. guajava.

    Item Type: Article
    Creators:
    1. Razak, F.A.
    2. Yusoff, M.
    3. Rahim, Z.H.A.
    Journal or Publication Title: Pakistan Journal of Medical Sciences
    Additional Information: ISI Document Delivery No.: 574ZX Times Cited: 1 Cited Reference Count: 21 Cited References: Fathilah A R, 2009, Pak J Biol Sci, V12, P518, DOI 10.3923/pjbs.2009.518.521 GRIFFITHS PL, 2008, LETT APPL MICROBIOL, V17, P152 Nalina T., 2007, American Journal of Biochemistry and Biotechnology, V3, P10 Fathilah A. R., 2007, Research Journal of Microbiology, V2, P381 Nalina T., 2006, Pakistan Journal of Biological Sciences, V9, P1470 FATHILAH AR, 2006, J ORAL SCI, V48, P71 SAMANARAYAKE LP, 2006, ESSENTIAL MICROBIOLO FATHILAH AR, 2005, THESIS U MALAYA FATHILAH AR, 2003, J ORAL SCI, V45, P201 MCCALL D, 2001, INTRO MICROBIOLOGY 1, P37 INDU BJ, 2000, HERBS GREEN PHARM MA Marsh PD, 1999, DENT PLAQUE REVISITE FATHILAH AR, 1999, ANN DENT, V6, P17 HOBERT I, 1998, GUAVA MED SAFE CHEAP Jones CG, 1997, PERIODONTOL 2000, V15, P55, DOI 10.1111/j.1600-0757.1997.tb00105.x Nisengard RJ, 1994, ORAL MICROBIOLOGY IM Scheie A A, 1994, Adv Dent Res, V8, P246 Ponglux D., 1987, MED PLANTS Gerhardt P, 1981, MANUAL METHODS GEN B Clark PF, 1919, J BACTERIOL, V4, P615 Todar K., TODARS ONLINE TXB BA Fathilah, A. R. Yusoff, M. Rahim, Z. H. A. Malaysian GovernmentIRPA 09-02-03-0197-EA197; University of MalayaF0392/2004B This research was financially supported by the IRPA 09-02-03-0197-EA197 Grant from the Malaysian Government and Vote F0392/2004B from the University of Malaya. Professional medical publications Saddar Part 2
    Uncontrolled Keywords: Psidium Guajava Piper Betle Bacterial Ultra-Structural Alteration Anti-Plaque Activity
    Subjects: R Medicine > RK Dentistry
    Divisions: Faculty of Dentistry > Dept of Oral Biology
    Depositing User: Mr Ahmad Azwan Azman
    Date Deposited: 14 Jun 2012 12:26
    Last Modified: 14 Jun 2012 12:26
    URI: http://eprints.um.edu.my/id/eprint/3305

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