Piper betle L. and Psidium guajava L. in oral health maintenance

Fathilah, A.R. (2011) Piper betle L. and Psidium guajava L. in oral health maintenance. Journal of Medicinal Plants Research, 5 (2). pp. 156-163. ISSN 1996-0875


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The objective of the study was to seek for new agents from plant extracts for use in dental plaque control. The emphasis was directed at the initial stage of dental plaque formation, specifically the adherence of bacteria referred to as the early plaque colonisers, to the acquired pellicle on the supragingival tooth surface. Streptococcus sanguinis, Streptococcus mitis and Actinomyces sp., the predominant bacteria involved at this initial stage of plaque development were used as test organisms. The aqueous extracts of Piper betle and Psidium guajava exhibited antimicrobial activities with MIC values in the range of 2.61 to 4.69 mg/mL and toxicity values (LC(50) and EC(50)) well above their toxic concentrations. P. betle and P. guajava extracts contained 9.25 and 11.5 ppm fluoride, respectively. Some aggregating activity was also displayed by the extract of the latter. Both extracts showed positive antiadherence activity and reduced the cell-surface hydrophobicity of the bacteria which might have rendered them less adherent and hence, minimising their adhesion to the tooth surface during the early stage of plaque development. Both extracts also suppressed the growth of these bacteria. Such an activity was reaffirmed and supported by SEM micrographs whereby the bacterial cells were unable to divide or grow successfully and hence suggesting bacteriostatic effect of the extracts. Extracts of P. betle and P. guajava may work by first preventing and reducing the adhesion of primary bacterial colonisers to the tooth surface and second, to prevent or inhibit the growth and proliferation of microorganisms adhering on to the tooth surface and may potentiate their antiplaque activities.

Item Type: Article
Additional Information: ISI Document Delivery No.: 729HI Times Cited: 0 Cited Reference Count: 28 Cited References: FATHILAH AR, 2010, J MED PLANT IN PRESS PALOMBO EA, 2009, ECAM, P1 SAMY J, 2009, HERBS MALAYSIA, P11 Fathilah A R, 2009, Pak J Biol Sci, V12, P518, DOI 10.3923/pjbs.2009.518.521 FATHILAH AR, 2009, PAK J MED SCI, V25, P926 OBINNA NC, 2008, J MED PLANTS RES, V2, P189 Prabu GR, 2006, J APPL MICROBIOL, V101, P487, DOI 10.1111/j.1365-2672.2006.02912.x FATHILAH AR, 2006, J ORAL SCI, V48, P71 FATHILAH AR, 2003, J ORAL SCI, V45, P201 HOBERT I, 2001, GUAVA MED SAFE CHEAP, P1 Koo H, 2000, ARCH ORAL BIOL, V45, P141, DOI 10.1016/S0003-9969(99)00117-X INDU BJ, 2000, HERBS GREEN PHARM MA, P81 Matsumoto M, 1999, CARIES RES, V33, P441, DOI 10.1159/000016549 Jones CG, 1997, PERIODONTOL 2000, V15, P55, DOI 10.1111/j.1600-0757.1997.tb00105.x Lagerlof F, 1994, Adv Dent Res, V8, P229 CUMMINS D, 1992, CLIN BIOL ASPECTS DE, P205 ADDY M, 1992, CLIN BIOL ASPECTS DE, P249 CUMMINS D, 1991, BIOFOULING, V4, P199 KOGA T, 1990, INFECT IMMUN, V58, P289 Hamilton I R, 1990, J Dent Res, V69 Spec No, P660 PONGLUX D, 1987, MED PLANTS, P209 JENKINSON HF, 1986, J GEN MICROBIOL, V132, P1575 SMITH JR, 1985, LEARNING GUIDES MED, P377 MCBRIDE BC, 1984, INFECT IMMUN, V44, P68 GIBBONS RJ, 1983, INFECT IMMUN, V41, P1190 MEYER BN, 1982, PLANTA MED, V45, P31, DOI 10.1055/s-2007-971236 MALTZ M, 1982, J DENT RES, V61, P786, DOI 10.1177/00220345820610062701 LIGHT TS, 1975, J CHEM EDUC, V52, P247 Fathilah, A. R. University of Malaya I would like to acknowledge Prof. Zubaidah Hj Abdul Rahim and Prof. Yasmin Othman who have supervised me in this PhD project and Assoc. Prof. Md. Yusoff Musa for his help in the electron microscopy work. The research was financially supported by the Vote F fund from the University of Malaya. Academic journals Victoria island
Uncontrolled Keywords: Dental plaque experimental pellicle antiadherence cell-surface hydrophobicity streptococcus-mutans hydrophobicity adherence bacteria fluoride
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dept of Oral Biology
Depositing User: Mr Ahmad Azwan Azman
Date Deposited: 14 Jun 2012 07:23
Last Modified: 14 Jun 2012 07:23
URI: http://eprints.um.edu.my/id/eprint/3303

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