Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243

Arzmi, M.H.; Razak, F.A.; Musa, M.Y.; Harun, W. (2012) Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243. Fems Yeast Research, 12 (3). pp. 351-358. ISSN 1567-1356

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    Abstract

    Phenotypic switching is characterized as a virulence factor of Candida spp. This study was carried out to evaluate the phenotypic switching ability of C. krusei ATCC 14243 and to determine its effect on the biological properties, adherence capacity and susceptibility towards chlorhexidine digluconate (CHX). To induce switched generations C. krusei was cultured under nitrogen-depleted growth conditions by adding phloxine B. These phenotypically switched colonies were designated as the 1st generation. Subsequent sub-culturing was performed to produce the 2nd, 3rd and 4th switched generations. The recovery of the 3rd generation was the highest at 85.7 while that of the 4th generation was lower at 70.8, and the recovery of the 1st and 2nd generations gradually reduced to 46.6 and 36.4, respectively. All generations of C. krusei were susceptible towards CHX. The unswitched C. krusei was the most susceptible but the least adherent to coated hard surfaces. The 2nd generation was the least susceptible, but with the highest adherent ability. The minimum inhibition concentration and minimal fungicidal concentration of C. krusei of all generations were determined at 0.4 mg mL(-1). These observations suggest that the switching activity of C. krusei induces changes to its biological properties and susceptibility towards CHX.

    Item Type: Article
    Creators:
    1. Arzmi, M.H.
    2. Razak, F.A.
    3. Musa, M.Y.
    4. Harun, W.
    Journal or Publication Title: Fems Yeast Research
    Additional Information: ISI Document Delivery No.: 923HZ Times Cited: 0 Cited Reference Count: 35 Cited References: Jain N, 2009, CELL MICROBIOL, V11, P1716, DOI 10.1111/j.1462-5822.2009.01384.x Alby K, 2009, MOL BIOL CELL, V20, P3178, DOI 10.1091/mbc.E09-01-0040 Pusateri CR, 2009, ARCH ORAL BIOL, V54, P588, DOI 10.1016/j.archoralbio.2009.01.016 Rahim ZHA, 2008, RES J MICROBIOLOGY, V3, P466 Wan Nordini Hasnor WI, 2007, THESIS U MALAYA MALA Pelletier R, 2005, MED MYCOL, V43, P559, DOI 10.1080/13693780500220415 Laffey SF, 2005, MICROBIOL-SGM, V151, P1073, DOI 10.1099/mic.0.27739-0 Cappucino J., 2005, MICROBIOLOGY LAB MAN Veerman ECI, 2004, BIOCHEM J, V381, P447 Bonacorsi C, 2004, BRAZ J MED BIOL RES, V37, P207 Vargas KG, 2004, MED SCI MONITOR, V10, P198 Imbert C, 2003, J ANTIMICROB CHEMOTH, V51, P281, DOI 10.1093/jac/dkg088 Freitas CS, 2003, PESQ ODONTOL BRAS, V17, P78 Lackhe SA, 2002, MICROBIOLOGY, V148, P2661 Haynes K, 2001, TRENDS MICROBIOL, V9, P591, DOI 10.1016/S0966-842X(01)02237-5 Coote JG, 2001, ADV MICROB PHYSIOL, V44, P141, DOI 10.1016/S0065-2911(01)44013-6 Abbas J, 2000, ARCH INTERN MED, V160, P2659, DOI 10.1001/archinte.160.17.2659 Lackhe SA, 2000, INFECT IMMUNE, V68, P884 Samaranayake L. P., 1997, Hong Kong Medical Journal, V3, P312 JONES S, 1994, J CLIN MICROBIOL, V32, P2869 SAMARANAYAKE YH, 1994, J MED MICROBIOL, V41, P295 ODDS FC, 1994, J AM ACAD DERMATOL, V31, pS2, DOI 10.1016/S0190-9622(08)81257-1 SCULLY C, 1994, CRIT REV ORAL BIOL M, V5, P125 SOLL DR, 1992, CLIN MICROBIOL REV, V5, P183 ODDS FC, 1989, MYCOSES, V32, P275 Odds FC, 1988, CANDIDA CANDIDOSIS R ANDERSON JM, 1987, J BACTERIOL, V169, P5579 SOLL DR, 1987, J CLIN MICROBIOL, V25, P1611 SLUTSKY B, 1987, J BACTERIOL, V169, P189 ANDERSON JM, 1986, J GEN MICROBIOL, V132, P2035 SLUTSKY B, 1985, SCIENCE, V230, P666, DOI 10.1126/science.3901258 POMES R, 1985, J GEN MICROBIOL, V131, P2107 Barnett JA, 1983, YEAST CHARACTERISTIC KURTZMAN CP, 1980, INT J SYST BACTERIOL, V30, P503 MIDDELHOVEN WJ, 1976, J BACTERIOL, V128, P851 Arzmi, Mohd Hafiz Razak, Fathilah Abdul Musa, Md Yusoff Harun, Wan Himratul Aznita Wan Fp011/2006a; rufs010/2007a; umrg095/09 htm; mosti; ipppps070/2009a This work was funded by FP 011/2006A, RUFS 010/2007A, UMRG 095/09 HTM, MOSTI and IPPP grant PS070/2009A and assisted by Mr Roslee Halip. Wiley-blackwell Malden
    Uncontrolled Keywords: Susceptibility Adherence Saliva-Coated Surfaces White-Opaque Transition Albicans Infections Mutants Strains Cells
    Subjects: R Medicine > RK Dentistry
    Divisions: Faculty of Dentistry > Dept of Oral Biology
    Depositing User: Mr Ahmad Azwan Azman
    Date Deposited: 14 Jun 2012 12:24
    Last Modified: 14 Jun 2012 12:24
    URI: http://eprints.um.edu.my/id/eprint/3302

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