Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli

Fong, M.Y.; Lau, Y.L.; Init, I.; Jamaiah, I.; Anuar, A.K.; Rahmah, N. (2003) Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli. The Southeast Asian Journal of Tropical Medicine and Public Health, 34 (4). pp. 723-726. ISSN 0125-1562

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    Abstract

    The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES- 120 antigen produced by the bacterium was investigated.A total of 45 human serum samples from patients infected with different helminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.

    Item Type: Article
    Creators:
    1. Fong, M.Y.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    2. Lau, Y.L.
    3. Init, I.
    4. Jamaiah, I.
    5. Anuar, A.K.
    6. Rahmah, N.
    Journal or Publication Title: The Southeast Asian Journal of Tropical Medicine and Public Health
    Additional Information: Correspondence: Dr Mun-Yik Fong, Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Fax: 603-79674754 E-mail: fongmy@um.edu.my
    Uncontrolled Keywords: Parasitology
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Fong Mun Yik
    Date Deposited: 13 Jun 2012 09:36
    Last Modified: 13 Jun 2012 09:36
    URI: http://eprints.um.edu.my/id/eprint/3275

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