Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli

Fong, Mun Yik and Lau, Y.L. and Ithoi, Init and Jamaiah, I. and Anuar, A.K. and Rahmah, N. (2003) Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli. The Southeast Asian Journal of Tropical Medicine and Public Health, 34 (4). pp. 723-726. ISSN 0125-1562,

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Abstract

The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES- 120 antigen produced by the bacterium was investigated.A total of 45 human serum samples from patients infected with different helminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Correspondence: Dr Mun-Yik Fong, Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Fax: 603-79674754 E-mail: fongmy@um.edu.my
Uncontrolled Keywords:	Parasitology
Subjects:	R Medicine
Divisions:	Faculty of Medicine
Depositing User:	Fong Mun Yik
Date Deposited:	13 Jun 2012 01:36
Last Modified:	30 Dec 2021 08:29
URI:	http://eprints.um.edu.my/id/eprint/3275

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