Liu, Yi and Kong, Kin Weng and Wu, Ding-Tao and Liu, Hong-Yan and Li, Hua-Bin and Zhang, Jia-Rong and Gan, Ren-You (2022) Pomegranate peel-derived punicalagin: Ultrasonic-assisted extraction, purification, and its alpha-glucosidase inhibitory mechanism. Food Chemistry, 374. ISSN 0308-8146, DOI https://doi.org/10.1016/j.foodchem.2021.131635.
Full text not available from this repository.Abstract
The pomegranate peel is a by-product of pomegranate fruit rich in polyphenols. In this study, pomegranate peel polyphenols were explored using LC-MS/MS, and punicalagin was the most abundant compound. The highest yield (505.89 +/- 1.73 mg/g DW) of punicalagin was obtained by ultrasonic-assisted extraction (UAE) with the ethanol concentration of 53%, sample-to-liquid ratio of 1:25 w/v, ultrasonic power of 757 W, and extraction time of 25 min. Punicalagin was further purified by the macroporous resin D101 and prep-HPLC, reaching the purity of 92.15%. The purified punicalagin had the IC50 of 82 +/- 0.02 mu g/mL against alpha-glucosidase, similar to the punicalagin standard with IC50 of 58 +/- 0.014 mu g/mL, both exhibiting a mixed inhibitory mechanism. Molecular docking further revealed that a steric hindrance with the intermolecular energy of - 7.99 kcal/mol was formed between punicalagin and alpha-glucosidase. Overall, pomegranate peel is a promising source of punicalagin to develop anti-diabetic functional foods.
| Item Type: | Article |
|---|---|
| Funders: | Local Financial Funds of National Agricultural Science and Technology Center, Chengdu [NASC2020KR02], Central Public-interest Scientific Institution Basal Research Fund [Y2020XK05] |
| Uncontrolled Keywords: | Polyphenols; Ultrasonic-assisted extraction; LC-MS/MS; Alpha-Glucosidase; Inhibition kinetic; Molecular docking |
| Subjects: | Q Science > QC Physics Q Science > QP Physiology R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine T Technology > TP Chemical technology T Technology > TX Home economics |
| Divisions: | Faculty of Medicine > Medicine Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 11 Aug 2022 00:59 |
| Last Modified: | 11 Aug 2022 00:59 |
| URI: | http://eprints.um.edu.my/id/eprint/32728 |
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